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Identification and Analysis of the Transcriptional Regulatory Networks Governing Mechanosensitive Channels in E. coli

Barnes, Stephanie and Jones, Daniel and Belliveau, Nathan and Sundaresh, Sushant and Kinney, Justin and Phillips, Rob (2014) Identification and Analysis of the Transcriptional Regulatory Networks Governing Mechanosensitive Channels in E. coli. Biophysical Journal, 106 (2). 487A. ISSN 0006-3495. http://resolver.caltech.edu/CaltechAUTHORS:20140710-104425641

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Abstract

Mechanosensitive (MS) channels are membrane proteins which are gated by mechanical stress in the cell membrane. Channel opening in response to mechanical stresses makes it possible for molecules to pass through the membrane, allowing an organism to alleviate solute imbalances which place osmotic stress on the membrane and can cause cell lysis and death. Two of the seven known MS channels in E. coli, MscL and MscS, are individually capable of rescuing these bacteria from sudden changes in osmolarity (osmotic shock). If MscL and MscS are both sufficient for ensuring survival under osmotic shock conditions, what is the purpose of the other five MS channels? In the interest of exploring the physiological roles of the various MS channels, we hypothesize that understanding how they are regulated will shed light on their function. Specifically, we investigate the regulatory context of the MS genes by identifying the regulatory architecture and transcription factors responsible for controlling MS gene output. We use a method known as "Sort-Seq" to locate transcription factor binding sites with base pair resolution, and DNA affinity chromatography to determine transcription factor identity. Understanding how the MS genes are regulated will give us clues as to the specific stressors which they defend the cell against. This work provides a specific case study of a method which we think will have much broader reach in mapping out regulatory networks genome-wide.


Item Type:Article
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http://dx.doi.org/10.1016/j.bpj.2013.11.4472DOIArticle
http://www.sciencedirect.com/science/article/pii/S0006349513057317PublisherArticle
http://www.biophysics.org/2014meeting/Main/tabid/4177/Default.aspxOrganizationConference Website
Additional Information:© 2014 Biophysical Society. Published by Elsevier Inc.
Record Number:CaltechAUTHORS:20140710-104425641
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140710-104425641
Official Citation:Stephanie Barnes, Daniel Jones, Nathan Belliveau, Sushant Sundaresh, Justin Kinney, Rob Phillips, Identification and Analysis of the Transcriptional Regulatory Networks Governing Mechanosensitive Channels in E. coli, Biophysical Journal, Volume 106, Issue 2, Supplement 1, 28 January 2014, Page 487a, ISSN 0006-3495, http://dx.doi.org/10.1016/j.bpj.2013.11.4472. (http://www.sciencedirect.com/science/article/pii/S0006349513057317)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:47139
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 Jul 2014 19:30
Last Modified:10 Jul 2014 19:30

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