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Direct phylogenetic and isotopic evidence for multiple groups of archaea involved in the anaerobic oxidation of methane

Orphan, V. J. and House, C. H. and Hinrichs, K.-U. and McKeegan, K. D. and DeLong, E. F. (2002) Direct phylogenetic and isotopic evidence for multiple groups of archaea involved in the anaerobic oxidation of methane. Geochimica et Cosmochimica Acta, 66 (S1). A571. ISSN 0016-7037. https://resolver.caltech.edu/CaltechAUTHORS:20140715-170201332

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Abstract

The biological oxidation of methane by anaerobic microorganisms is a significant sink for methane in the marine environment. Although there is convincing biogeochemical evidence for anaerobic oxidation of methane (AOM) by methanotrophic archaea and sulfate-reducing bacteria, the identity of these uncultured microorganisms is only now being described. In this study, we examined the diversity archaeal and bacterial assemblages involved in AOM using directly coupled isotopic and phylogenetic analyses at the level of single cells. The combined application of fluorescent in situ hybridization and secondary ion mass spectrometry (FISHSIMS) identified two phylogenetically distinct groups of archaea (ANME-1 and ANME-2) from marine methane seeps that were extremely depleted in carbon-13 (-83‰) and appear to be capable of directly oxidizing methane. These archaeal groups were observed to exist as monospecies aggregates or single cells as well as in physical association with bacteria including, but not limited to, members of the sulfate-reducing Desulfosarcina. The results from this work illustrate the complexity of the microbial communities and possible mechanisms involved in AOM. FISH-SIMS is an effective approach for understanding the dynamic microbial interactions within diverse methane-associated communities and may provide a useful culture-independent tool for deciphering the metabolic function of other environmentally significant microorganisms in situ.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0016-7037(02)01016-5DOIAbstracts
http://www.sciencedirect.com/science/article/pii/S0016703702010165PublisherAbstracts
http://goldschmidt.info/2002/programme/Programme.pdfOrganizationConference Website
ORCID:
AuthorORCID
Orphan, V. J.0000-0002-5374-6178
House, C. H.0000-0002-4926-4985
Hinrichs, K.-U.0000-0002-0739-9291
Additional Information:Copyright © 2002 Elsevier.
Issue or Number:S1
Record Number:CaltechAUTHORS:20140715-170201332
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140715-170201332
Official Citation:Abstracts, Geochimica et Cosmochimica Acta, Volume 66, Supplement 1, 15 August 2002, Pages 501-600, ISSN 0016-7037, http://dx.doi.org/10.1016/S0016-7037(02)01016-5
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:47249
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:16 Jul 2014 16:37
Last Modified:09 Mar 2020 13:18

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