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Structure of transcriptionally active chromatin

Gottesfeld, Joel M. and Murphy, Robert F. and Bonner, James (1975) Structure of transcriptionally active chromatin. Proceedings of the National Academy of Sciences of the United States of America, 72 (11). pp. 4404-4408. ISSN 0027-8424.

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Rat-liver chromatin has been fractionated into transcriptionally active and inactive regions [Gottesfeld et al. (1974) Proc. Nat. Acad. Sci. USA 71, 2193-2197] and the distribution of nuclease-resistant complexes in these fractions has been investigated. About half of the DNA of both fractions is resistant to attack by the endonuclease DNase II. The nuclease-resistant structures of inactive chromatin are DNA-histone complexes (v-bodies) which sediment at 11-13 S. Template-active chromatin yields two peaks of nuclease-resistant nucleoprotein. These complexes sediment at 14 and 19 S, and contain DNA, RNA, histone, and nonhistone chromosomal proteins. Polyacrylamide gel electrophoresis reveals a complex pattern of chromatin proteins, suggesting that the complexes are heterogeneous in composition.

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Additional Information:Copyright © 1975 by the National Academy of Sciences. Contributed by James Bonner, September 10, 1975. We wish to thank Dr. K. Peters and Mr. W. Wheatley for helpful discussions. This work was supported by the U.S. Public Health Service (GM 86 and GM 13762).
Subject Keywords:chromatin fractionation; deoxyribonuclease II; chromatin subunits; v-bodies)
Issue or Number:11
Record Number:CaltechAUTHORS:GOTpnas75
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4732
Deposited By: Tony Diaz
Deposited On:05 Sep 2006
Last Modified:02 Oct 2019 23:15

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