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The ties that bind: Dynamics of syntrophic associations in marine methane seeps

Orphan, Victoria J. and Pernthaler, Annelie and Dekas, Anne E. and Gammon, Crystal and House, Christopher H. (2007) The ties that bind: Dynamics of syntrophic associations in marine methane seeps. Geochimica et Cosmochimica Acta, 71 (15, S). A743. ISSN 0016-7037. https://resolver.caltech.edu/CaltechAUTHORS:20140723-162327312

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Abstract

The deep-sea methane seep environment supports active and diverse microbial assemblages supported by the anaerobic oxidation of methane (AOM). Unknown to science less than a decade ago, the microorganisms and the molecular mechanisms underlying this enigmatic and globally important biogeochemical process have been the subject of intensive study worldwide. The identification, activity, distribution, and partial metabolic pathway reconstruction of methanotrophic archaea and co-associated sulfate reducing bacteria has been characterized. However fundamental questions still remain regarding the necessity of a physically coupled syntrophic association between sulfate reducing bacteria and methane oxidizing archaea, the underlying biochemistry enabling sulfate-coupled methane oxidation, and the extent of the diversity of microbial assemblages involved in AOM. Using microanalytical stable isotope analyses of whole cells in tandem with genomics enabled molecular methods, we examined the variation in metabolic activity between individual aggregations of microorganisms recovered from methane seep sediments. Significant differences in activity were observed between archaeal-bacterial associations and mono-specific aggregations of putative methanotrophic archaea and sulfate-reducing populations, supporting enhanced metabolism in multi-species aggregates. Application of a new SSU rRNA targeted method for capturing and concentrating specific uncultured microbial populations from methane seep sediments has uncovered novel partnerships and additional insights into the metabolic potential of the methanotrophic archaea and co-associated bacteria.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.gca.2007.06.023DOIAbstracts
http://www.sciencedirect.com/science/article/pii/S0016703707003249PublisherAbstracts
http://goldschmidt.info/2007/index.phpOrganizationConference Website
ORCID:
AuthorORCID
Orphan, Victoria J.0000-0002-5374-6178
Dekas, Anne E.0000-0001-9548-8413
Additional Information:Copyright © 2007 Elsevier.
Issue or Number:15, S
Record Number:CaltechAUTHORS:20140723-162327312
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140723-162327312
Official Citation:Goldschmidt Abstracts 2007- O, Geochimica et Cosmochimica Acta, Volume 71, Issue 15, Supplement, August 2007, Pages A727-A746, ISSN 0016-7037, http://dx.doi.org/10.1016/j.gca.2007.06.023. (http://www.sciencedirect.com/science/article/pii/S0016703707003249)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:47447
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:24 Jul 2014 15:38
Last Modified:03 Oct 2019 20:00

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