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Cellular uptake and cytotoxicity of a near-IR fluorescent corrole–TiO_2 nanoconjugate

Blumenfeld, Carl M. and Sadtler, Bryce F. and Fernandez, G. Esteban and Dara, Lily and Nguyen, Cathie and Alonso-Valenteen, Felix and Medina-Kauwe, Lali and Moats, Rex A. and Lewis, Nathan S. and Grubbs, Robert H. and Gray, Harry B. and Sorasaenee, Karn (2014) Cellular uptake and cytotoxicity of a near-IR fluorescent corrole–TiO_2 nanoconjugate. Journal of Inorganic Biochemistry, 140 . pp. 39-44. ISSN 0162-0134. PMCID PMC4998051. https://resolver.caltech.edu/CaltechAUTHORS:20140729-072109271

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Abstract

We are investigating the biological and biomedical imaging roles and impacts of fluorescent metallocorrole–TiO_2 nanoconjugates as potential near-infrared optical contrast agents in vitro in cancer and normal cell lines. The TiO_2 nanoconjugate labeled with the small molecule 2,17-bis(chlorosulfonyl)-5,10,15-tris(pentafluorophenyl)corrolato aluminum(III) (1-Al–TiO_2) was prepared. The nanoparticle 1-Al–TiO_2 was characterized by transmission electron microscopy (TEM) and integrating-sphere electronic absorption spectroscopy. TEM images of three different samples of TiO_2 nanoparticles (bare, H_2O_2 etched, and 1-Al functionalized) showed similarity in shapes and sizes with an average diameter of 29 nm for 1-Al–TiO_2. Loading of 1-Al on the TiO_2 surfaces was determined to be ca. 20–40 mg 1-Al/g TiO_2. Confocal fluorescence microscopy (CFM) studies of luciferase-transfected primary human glioblastoma U87-Luc cells treated with the nanoconjugate 1-Al–TiO_2 as the contrast agent in various concentrations were performed. The CFM images revealed that 1-Al–TiO_2 was found inside the cancer cells even at low doses (0.02–2 μg/mL) and localized in the cytosol. Bioluminescence studies of the U87-Luc cells exposed to various amounts of 1-Al–TiO_2 showed minimal cytotoxic effects even at higher doses (2–2000 μg/mL) after 24 h. A similar observation was made using primary mouse hepatocytes (PMH) treated with 1-Al–TiO_2 at low doses (0.0003–3 μg/mL). Longer incubation times (after 48 and 72 h for U87-Luc) and higher doses (> 20 μg/mL 1-Al–TiO_2 for U87-Luc and > 3 μg/mL 1-Al–TiO_2 for PMH) showed decreased cell viability.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.jinorgbio.2014.06.015DOIArticle
http://www.sciencedirect.com/science/article/pii/S0162013414001767PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4998051/PubMed CentralArticle
ORCID:
AuthorORCID
Lewis, Nathan S.0000-0001-5245-0538
Grubbs, Robert H.0000-0002-0057-7817
Gray, Harry B.0000-0002-7937-7876
Alternate Title:Cellular uptake and cytotoxicity of a near-IR fluorescent corrole–TiO2 nanoconjugate
Additional Information:© 2014 Elsevier Inc. Received 13 March 2014, Revised 19 June 2014, Accepted 20 June 2014, Available online 28 June 2014. We thank the CHLA Radiology Endowment Fund (K.S.), Sanofi (H.B.G.), Doheny Eye Institute (R.H.G.), and Beckman Institute Postdoctoral Fellowship (B.F.S.) for the support. We also thank Anahit Hovsepyan, Seda Mkhitaryan, Vazgen Khankaldyyan, and Gevorg Karapetyan for the help with the bioluminescence assays. Absorption measurements and profilometry were performed at the Molecular Materials Research Center, Beckman Institute, Caltech. We thank Carol M. Garland for the assistance in TEM imaging. B.F.S. and N.S.L. acknowledge the support from the “Light–Material Interactions in Energy Conversion” Energy Frontier Research Center funded by the US Department of Energy, Office of Science, Office of Basic Energy Sciences (DE-SC0001293).
Funders:
Funding AgencyGrant Number
CHLA Radiology Endowment FundUNSPECIFIED
SanofiUNSPECIFIED
Doheny Eye InstituteUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Department of Energy (DOE)DE-SC0001293
Subject Keywords:Nanoconjugate; TiO2 nanoparticle; Corrole; Fluorescence; Cytotoxic effect; Cellular uptake
PubMed Central ID:PMC4998051
Record Number:CaltechAUTHORS:20140729-072109271
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140729-072109271
Official Citation:Carl M. Blumenfeld, Bryce F. Sadtler, G. Esteban Fernandez, Lily Dara, Cathie Nguyen, Felix Alonso-Valenteen, Lali Medina-Kauwe, Rex A. Moats, Nathan S. Lewis, Robert H. Grubbs, Harry B. Gray, Karn Sorasaenee, Cellular uptake and cytotoxicity of a near-IR fluorescent corrole–TiO2 nanoconjugate, Journal of Inorganic Biochemistry, Volume 140, November 2014, Pages 39-44, ISSN 0162-0134, http://dx.doi.org/10.1016/j.jinorgbio.2014.06.015. (http://www.sciencedirect.com/science/article/pii/S0162013414001767)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:47541
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:29 Jul 2014 14:47
Last Modified:22 Nov 2019 09:58

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