CaltechAUTHORS
  A Caltech Library Service

Evolution of a Pathway to Novel Long-Chain Carotenoids

Umeno, Daisuke and Arnold, Frances H. (2004) Evolution of a Pathway to Novel Long-Chain Carotenoids. Journal of Bacteriology, 186 (5). pp. 1531-1536. ISSN 0021-9193. PMCID PMC344396. http://resolver.caltech.edu/CaltechAUTHORS:UMEjbact04

[img]
Preview
PDF - Published Version
See Usage Policy.

807Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:UMEjbact04

Abstract

Using methods of laboratory evolution to force the C30 carotenoid synthase CrtM to function as a C40 synthase, followed by further mutagenesis at functionally important amino acid residues, we have discovered that synthase specificity is controlled at the second (rearrangement) step of the two-step reaction. We used this information to engineer CrtM variants that can synthesize previously unknown C45 and C50 carotenoid backbones (mono- and diisopentenylphytoenes) from the appropriate isoprenyldiphosphate precursors. With this ability to produce new backbones in Escherichia coli comes the potential to generate whole series of novel carotenoids by using carotenoid-modifying enzymes, including desaturases, cyclases, hydroxylases, and dioxygenases, from naturally occurring pathways.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC344396/PubMed CentralArticle
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2004, American Society for Microbiology. Received 5 June 2003/ Accepted 30 October 2003 This research was supported by the U.S. National Science Foundation (BES-0118565) and Maxygen, Inc.
Funders:
Funding AgencyGrant Number
NSFBES-0118565
Maxygen, Inc.UNSPECIFIED
PubMed Central ID:PMC344396
Record Number:CaltechAUTHORS:UMEjbact04
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:UMEjbact04
Alternative URL:http://dx.doi.org/10.1128/JB.186.5.1531-1536.2004
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4834
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:11 Sep 2006
Last Modified:12 Sep 2017 20:09

Repository Staff Only: item control page