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Evolution of a Pathway to Novel Long-Chain Carotenoids

Umeno, Daisuke and Arnold, Frances H. (2004) Evolution of a Pathway to Novel Long-Chain Carotenoids. Journal of Bacteriology, 186 (5). pp. 1531-1536. ISSN 0021-9193. PMCID PMC344396.

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Using methods of laboratory evolution to force the C30 carotenoid synthase CrtM to function as a C40 synthase, followed by further mutagenesis at functionally important amino acid residues, we have discovered that synthase specificity is controlled at the second (rearrangement) step of the two-step reaction. We used this information to engineer CrtM variants that can synthesize previously unknown C45 and C50 carotenoid backbones (mono- and diisopentenylphytoenes) from the appropriate isoprenyldiphosphate precursors. With this ability to produce new backbones in Escherichia coli comes the potential to generate whole series of novel carotenoids by using carotenoid-modifying enzymes, including desaturases, cyclases, hydroxylases, and dioxygenases, from naturally occurring pathways.

Item Type:Article
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Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2004, American Society for Microbiology. Received 5 June 2003/ Accepted 30 October 2003 This research was supported by the U.S. National Science Foundation (BES-0118565) and Maxygen, Inc.
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Issue or Number:5
PubMed Central ID:PMC344396
Record Number:CaltechAUTHORS:UMEjbact04
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4834
Deposited By: Archive Administrator
Deposited On:11 Sep 2006
Last Modified:02 Oct 2019 23:16

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