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A simple device for studying macromolecular crystals under moderate gas pressures (0.1-10 MPa)

Stowell, Michael H. B. and Soltis, S. Michael and Kisker, Caroline and Peters, John W. and Schindelin, Hermann and Rees, D. C. and Cascio, Duilio and Beamer, Lesa and Hart, P. John and Wiener, Michael C. and Whitby, Frank G. (1996) A simple device for studying macromolecular crystals under moderate gas pressures (0.1-10 MPa). Journal of Applied Crystallography, 29 (5). pp. 608-613. ISSN 0021-8898. doi:10.1107/S0021889896004712. https://resolver.caltech.edu/CaltechAUTHORS:20140812-125646031

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Abstract

A simple device for studying crystalline samples under moderate gas pressure (0.1-10 MPa) has been developed. The device employs a modified Cajon ultra-torr fitting to ensure a gas-tight seal around an X-ray capillary. The cell accommodates standard X-ray capillaries that require no modification. The device is straightforward to utilize and samples can be mounted with routine techniques and pressurized in a matter of seconds. In a subsequent development, a complete purging and pressurization system has been designed and constructed for use on beamline 7-1 at the Stanford Synchrotron Radiation Laboratory. This paper describes the construction of both the pressure cell and the delivery system and presents results of the use of this cell for the preparation of xenon derivatives to be used in phase determination by the multiple isomorphous replacement method.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://scripts.iucr.org/cgi-bin/paper?S0021889896004712PublisherArticle
http://dx.doi.org/10.1107/S0021889896004712 DOIArticle
ORCID:
AuthorORCID
Rees, D. C.0000-0003-4073-1185
Additional Information:© 1996 International Union of Crystallography. Received 12 December 1995; accepted 9 April 1996. This work was supported in part by NIH GM45062 of the NSF to OCR and by the DOE, Office of Basic Energy Sciences and the Office of Health and Environmental Research, and by the NIH, Biomedical Research Technology Program, National Center for Research Resources for support of the rotation camera facilities at SSRL. JPH is supported by a DOE Alexander Hollaender Distinguished Postdoctoral Fellowship. We wish to thank R. P. Phizackerley for assistance in the design of the gas-delivery system and Hartmut Luecke and Henry D. Bellamy for helpful discussions.
Funders:
Funding AgencyGrant Number
NIHGM45062
NSFUNSPECIFIED
NIHUNSPECIFIED
Department of Energy (DOE) Alexander Hollaender Distinguished Postdoctoral FellowshipUNSPECIFIED
Issue or Number:5
DOI:10.1107/S0021889896004712
Record Number:CaltechAUTHORS:20140812-125646031
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140812-125646031
Official Citation:J. Appl. Cryst. (1996). 29, 608-613 [ doi:10.1107/S0021889896004712 ] A simple device for studying macromolecular crystals under moderate gas pressures (0.1-10 MPa) M. H. B. Stowell, S. M. Soltis, C. Kisker, J. W. Peters, H. Schindelin, D. C. Rees, D. Cascio, L. Beamer, P. J. Hart, M. C. Wiener and F. G. Whitby
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:48398
Collection:CaltechAUTHORS
Deposited By:INVALID USER
Deposited On:12 Aug 2014 20:17
Last Modified:10 Nov 2021 18:31

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