CaltechAUTHORS
  A Caltech Library Service

Visualization of N-cadherin protein expression during the emigration of chicken trunk neural crest cells

Murray, Ben A. (2001) Visualization of N-cadherin protein expression during the emigration of chicken trunk neural crest cells. Molecular Biology of the Cell, 12 (S). 479A. ISSN 1059-1524. https://resolver.caltech.edu/CaltechAUTHORS:20140827-090853558

[img]
Preview
PDF - Published Version
Creative Commons Attribution Non-commercial Share Alike.

120Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20140827-090853558

Abstract

Emigration of trunk neural crest cells from the developing neural tube requires the breakage or rearrangement of cadherin-containing adherens junctions between the cells. I therefore examined the distribution of N-cadherin protein in chicken trunk neural crest cells as they emerged from the neural tube. To identify emerging neural crest cells, embryos were electroporated at the 12 to 16 somite stage of development with cDNA constructs encoding green or red fluorescent proteins and were allowed to develop for a further 12 to 24 hours before fixation. Sections or whole mounts then were stained with anti-cadherin antibodies and examined by conventional or confocal microscopy. As expected from previous work, fully-emigrated cells did not express detectable levels of surface N-cadherin. In some cases, cells that had not fully emerged from the neural tube appeared to retain surface-localized N-cadherin at areas of apposition to other neural tube cells. This suggests that modulation or breakage of N-cadherin-containing adherens junctions may be involved in control of neural crest emigration in the trunk. This work was supported by a Grant-in-Aid from the American Heart Association Western Regional Affiliates.


Item Type:Article
Additional Information:© 2001 American Society for Cell Biology. Under the License and Publishing Agreement, authors grant to the general public, effective two months after publication of (i.e.,. the appearance of) the edited manuscript in an online issue of MBoC, the nonexclusive right to copy, distribute, or display the manuscript subject to the terms of the Creative Commons–Noncommercial–Share Alike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0). This work was supported by a Grant-in-Aid from the American Heart Association Western Regional Affiliates.
Funders:
Funding AgencyGrant Number
American Heart Association Western Regional AffiliatesUNSPECIFIED
Issue or Number:S
Record Number:CaltechAUTHORS:20140827-090853558
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140827-090853558
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:48967
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:29 Aug 2014 22:00
Last Modified:03 Oct 2019 07:09

Repository Staff Only: item control page