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Studies on Nucleic Acid Reassociation Kinetics: Retarded Rate of Hybridization of RNA with Excess DNA

Galau, Glenn A. and Smith, Michael J. and Britten, Roy J. and Davidson, Eric H. (1977) Studies on Nucleic Acid Reassociation Kinetics: Retarded Rate of Hybridization of RNA with Excess DNA. Proceedings of the National Academy of Sciences of the United States of America, 74 (6). pp. 2306-2310. ISSN 0027-8424. PMCID PMC432159.

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The rate of reaction of excess double-stranded bacteriophage phi X174 and plasmid RSF2124 DNA drivers with enzymatically synthesized asymmetric RNA tracers was measured. Other reactions were carried out with excess Escherichia coli DNA and E. coli RNA labeled in vivo. RNA and DNA fragment lengths were held approximately equal. For each case it was shown that in DNA excess the rate constant for RNA· DNA hybridization is 3- to 4.5-fold lower than that of the renaturation rate constant for the driver DNA. This retardation was also observed in pseudo-first-order hybridization reactions driven by excess strand-separated RSF2124 DNA. It was concluded that the rate constant for RNA· DNA hybridization depends partially on which species is in excess.

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Additional Information:Copyright © 1977 by the National Academy of Sciences Contributed by Roy J. Britten, March 18, 1977 We gratefully acknowledge the gifts of φX174 nucleic acids by Drs. Lloyd H. Smith, Amy S. Lee, Paul H. Johnson, and Robert L. Sinsheimer. We thank Dr. Norman Davidson for his critical review of the manuscript. This research was supported by National Institutes of Health Grants HD-05753 and GM-20927 and by National Science Foundation Grant BMS 75-07359. The costs of publication of this article were defrayed in part by the payment of page charges from funds made available to support the research which is the subject of the article. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact. This is paper IV in a series. Paper III is ref. 1.
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NSFBMS 75-07359
Subject Keywords:plasmid RSF2124 nucleic acids; bacteriophage φX174 nucleic acids; Escherichia coli nucleic acids
Issue or Number:6
PubMed Central ID:PMC432159
Record Number:CaltechAUTHORS:GALpnas77b
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4907
Deposited By: Archive Administrator
Deposited On:12 Sep 2006
Last Modified:02 Oct 2019 23:17

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