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Cloning, sequencing, and mutation of a gene for azurin in Methylobacillus flagellatum KT

Gak, Evgeny R. and Chistoserdov, Andrei Y. and Lidstrom, Mary E. (1995) Cloning, sequencing, and mutation of a gene for azurin in Methylobacillus flagellatum KT. Journal of Bacteriology, 177 (15). pp. 4575-4578. ISSN 0021-9193.

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The gene cluster for methylamine utilization (mau genes) has been cloned from the obligate methylotrophic bacterium Methylobacillus flagellatum KT. Partial sequence data showed that the organization of these genes was similar to that found in Methylophilus methylotrophus W3A1-NS, including the lack of a gene for amicyanin, which had been thought to be the electron acceptor for methylamine dehydrogenase in M. flagellatum KT. However, a gene encoding azurin was discovered at the 3' end of the mau gene cluster, transcribed in the opposite orientation. A mutant with a defect in this gene showed impaired growth on methylamine, suggesting that azurin is involved in methylamine oxidation in M. flagellatum KT.

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Additional Information:Copyright © 1995, American Society for Microbiology Received 16 December 1994/Accepted 1 June 1995 This work was supported by a grant from the NIH (GM 36296). Nucleotide sequence accession numbers. The GenBank accession numbers for the nine partial or complete M. flagellatum KT open reading frames (mauFBEDAGLMN) that showed substantial similarity in the regions sequenced to their counterparts from M. methylotrophus W3A1-NS are L37426, L37427, L37428, L37429, L37433, L37434, L37435, and L37436.
Issue or Number:15
Record Number:CaltechAUTHORS:GAKjbact95
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ID Code:4910
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Deposited On:12 Sep 2006
Last Modified:02 Oct 2019 23:17

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