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Sequence Analysis of Three Sindbis Virus Mutants Temperature-Sensitive in the Capsid Protein Autoprotease

Hahn, Chang S. and Strauss, Ellen G. and Strauss, James H. (1985) Sequence Analysis of Three Sindbis Virus Mutants Temperature-Sensitive in the Capsid Protein Autoprotease. Proceedings of the National Academy of Sciences of the United States of America, 82 (14). pp. 4648-4652. ISSN 0027-8424. PMCID PMC390443.

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We have cloned and sequenced the cDNA made to the region of RNA encoding the structural proteins of three complementation group C mutants of Sindbis virus, ts2, ts5, and ts13, and of their revertants. These mutants possess defects in the posttranslational processing of their structural proteins at the nonpermissive temperature. Comparison of the deduced amino acid sequences of the mutants with those of the revertants and with the parental HR strain of virus showed all three mutants to have single amino acid substitutions in the highly conserved COOH-terminal half of the capsid protein that give rise to temperature sensitivity. ts2 and ts5 were found to have the same lesion and thus represent independent isolations of the same mutant, whereas ts13 possessed a different change. Reversion to temperature insensitivity in all three mutants occurred by reversion of the mutated nucleotide to the parental nucleotide, restoring the original amino acid. It has been previously postulated that the capsid protein possesses an autoproteolytic activity that cleaves the capsid protein from the nascent polyprotein during translation. Comparison of the amino acid sequence of the capsid protein with that of serine proteases leads us to hypothesize that histidine-141, aspartate-147, and serine-215 of the Sindbis capsid protein form the catalytic triad of a serine protease. This hypothesis is supported by the finding that all three temperature-sensitive lesions mapped occur near these residues: ts2 and ts5 change proline-218 to serine and in ts13 lysine-138 has been replaced by isoleucine.

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Additional Information:Copyright © 1985 by the National Academy of Sciences Communicated by Giuseppe Attardi, March 25, 1985 We thank E. Lenches for expert technical assistance in preparation of cells and of virus stocks and are grateful to Dr. Charles Rice for helpful discussions. This work was supported by Grants AI 10793 and AI 20612 from the National Institutes of Health and by Grant DMB 8316856 from the National Science Foundation. C.S.H. was supported by a grant from the California Foundation for Biochemical Research. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
NIHAI 10793
NIHAI 20612
California Foundation for Biochemical ResearchUNSPECIFIED
Subject Keywords:alphavirus; serine protease; RNA virus evolution
Issue or Number:14
PubMed Central ID:PMC390443
Record Number:CaltechAUTHORS:HAHpnas85
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4914
Deposited By: Archive Administrator
Deposited On:12 Sep 2006
Last Modified:02 Oct 2019 23:17

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