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The enzymatic synthesis of protein. III. The effect of the hydrogen ion concentration on peptic synthesis

Wasteneys, Hardolph and Borsook, Henry (1925) The enzymatic synthesis of protein. III. The effect of the hydrogen ion concentration on peptic synthesis. Journal of Biological Chemistry, 62 (3). pp. 675-686. ISSN 0021-9258. https://resolver.caltech.edu/CaltechAUTHORS:20140902-143116989

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Abstract

In two previous communications the authors have described a synthesis of protein by pepsin in a concentrated peptic hydrolysate of albumin (1); and the effect of temperature on this synthesis (2). The justification for describing the synthetic product as protein is discussed in our previous paper (2). In these communications the optimum hydrogen ion concentration was stated to be pH 4.0; but the influence of the hydrogen ion concentration was otherwise not discussed. The importance of the degree of acidity was realized early by Sawjalow (3), who did not, however, define it precisely, and by Henriques and Gjaldbäk (4), who gave the optimum pH as 1.5. This hydrogen ion concentration in our experience, despite the existence of all other optimum conditions, allows only very small amounts of synthesis. Either Henriques and Gjaldbäk were in error, or the occurrence of the optimum pH at 1.5 was due to some as yet unrecognized factor.


Item Type:Article
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http://www.jbc.org/content/62/3/675PublisherArticle
Additional Information:© 1925 American Society for Biochemistry and Molecular Biology. Received for publication, November 22, 1924. The authors desire to express their gratitude to Professor Andrew Hunter for his interest and helpful criticism.
Issue or Number:3
Record Number:CaltechAUTHORS:20140902-143116989
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20140902-143116989
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:49141
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Sep 2014 16:32
Last Modified:03 Oct 2019 07:11

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