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Fourier ptychographic microscopy for filtration-based circulating tumor cell enumeration and analysis

Williams, Anthony and Chung, Jaebum and Ou, Xiaoze and Zheng, Guoan and Rawal, Siddharth and Ao, Zheng and Datar, Ram and Yang, Changhuei and Cote, Richard (2014) Fourier ptychographic microscopy for filtration-based circulating tumor cell enumeration and analysis. Journal of Biomedical Optics, 19 (6). Art. No. 066007. ISSN 1083-3668. PMCID PMC4572097. http://resolver.caltech.edu/CaltechAUTHORS:20140912-103400081

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Abstract

Circulating tumor cells (CTCs) are recognized as a candidate biomarker with strong prognostic and predictive potential in metastatic disease. Filtration-based enrichment technologies have been used for CTC characterization, and our group has previously developed a membrane microfilter device that demonstrates efficacy in model systems and clinical blood samples. However, uneven filtration surfaces make the use of standard microscopic techniques a difficult task, limiting the performance of automated imaging using commercially available technologies. Here, we report the use of Fourier ptychographic microscopy (FPM) to tackle this challenge. Employing this method, we were able to obtain high-resolution color images, including amplitude and phase, of the microfilter samples over large areas. FPM’s ability to perform digital refocusing on complex images is particularly useful in this setting as, in contrast to other imaging platforms, we can focus samples on multiple focal planes within the same frame despite surface unevenness. In model systems, FPM demonstrates high image quality, efficiency, and consistency in detection of tumor cells when comparing corresponding microfilter samples to standard microscopy with high correlation (R^2=0.99932). Based on these results, we believe that FPM will have important implications for improved, high throughput, filtration-based CTC analysis, and, more generally, image analysis of uneven surfaces.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1117/1.JBO.19.6.066007DOIArticle
http://dx.doi.org/10.1117/1.JBO.20.12.129802ErrataErrata
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4572097PubMed CentralArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6001938PubMed CentralErrata
Additional Information:© 2014 The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. Paper 140142R received Mar. 4, 2014; revised manuscript received May 7, 2014; accepted for publication May 21, 2014; published online Jun. 20, 2014. The authors would like to thank all volunteers for graciously donating blood samples for the purpose of these studies. Funding for this work was provided through a Department of Defense grant award W81XWH-09-1-0050 (Principal Investigators: C. Yang and R. Cote), and the Sylvester Comprehensive Cancer Center at the University of Miami—Miller School of Medicine. Funding for A. Williams was provided through a fellowship award from the UNCF-Merck Science Initiative.
Funders:
Funding AgencyGrant Number
Department of Defense (DOD)W81XWH-09-1-0050
UNCF-Merck Science InitiativeUNSPECIFIED
University of Miami Miller School of MedicineUNSPECIFIED
Subject Keywords:metastasis; circulating tumor cells; Fourier ptychographic microscopy; microfilter device
PubMed Central ID:PMC4572097
Record Number:CaltechAUTHORS:20140912-103400081
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140912-103400081
Official Citation:Fourier ptychographic microscopy for filtration-based circulating tumor cell enumeration and analysis Anthony Williams; Jaebum Chung; Xiaoze Ou; Guoan Zheng; Siddarth Rawal; Zheng Ao; Ram Datar; Changhuei Yang; Richard Cote J. Biomed. Opt. 19 (6), 066007 (June 20, 2014); doi: 10.1117/1.JBO.19.6.066007
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:49658
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:12 Sep 2014 19:51
Last Modified:26 Jun 2018 19:45

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