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Dominance of misfolded intermediates in the dynamics of α-helix folding

Lin, Milo M. and Shorokov, Dmitry and Zewail, Ahmed H. (2014) Dominance of misfolded intermediates in the dynamics of α-helix folding. Proceedings of the National Academy of Sciences of the United States of America, 111 (40). pp. 14424-14429. ISSN 0027-8424. PMCID PMC4210006. http://resolver.caltech.edu/CaltechAUTHORS:20140923-113146972

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Abstract

Helices are the “hydrogen atoms” of biomolecular complexity; the DNA/RNA double hairpin and protein α-helix ubiquitously form the building blocks of life’s constituents at the nanometer scale. Nevertheless, the formation processes of these structures, especially the dynamical pathways and rates, remain challenging to predict and control. Here, we present a general analytical method for constructing dynamical free-energy landscapes of helices. Such landscapes contain information about the thermodynamic stabilities of the possible macromolecular conformations, as well as about the dynamic connectivity, thus enabling the visualization and computation of folding pathways and timescales. We elucidate the methodology using the folding of polyalanine, and demonstrate that its α-helix folding kinetics is dominated by misfolded intermediates. At the physiological temperature of T = 298 K and midfolding time t = 250 ns, the fraction of structures in the native-state (α-helical) basin equals 22%, which is in good agreement with time-resolved experiments and massively distributed, ensemble-convergent molecular-dynamics simulations. We discuss the prominent role of β-strand–like intermediates in flight toward the native fold, and in relation to the primary conformational change precipitating aggregation in some neurodegenerative diseases.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.1416300111DOIArticle
http://www.pnas.org/content/111/40/14424.abstractPublisherArticle
http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1416300111/-/DCSupplementalPublisherSupporting Information
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210006/PubMed CentralArticle
Additional Information:Copyright © 2014 National Academy of Sciences. Contributed by Ahmed H. Zewail, August 26, 2014 (sent for review August 1, 2014; reviewed by Martin Gruebele, David J. Wales, and Feng Gai) Published online before print September 22, 2014, doi: 10.1073/pnas.1416300111. We acknowledge the critical and helpful comments from experts in the field: Drs. William Eaton, Feng Gai, Martin Gruebele, and David Wales. We are grateful to the National Science Foundation for funding of this research. M.M.L. acknowledges financial support from the Krell Institute and the US Department of Energy (US Department of Energy Grant DE-FG02-97ER25308) for a graduate fellowship at Caltech. Author contributions: M.M.L., D.S., and A.H.Z. designed research, performed research, and wrote the paper. Reviewers: M.G., University of Illinois at Urbana–Champaign; D.J.W., Cambridge University; and F.G., University of Pennsylvania. The authors declare no conflict of interest. This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1416300111/-/DCSupplemental.
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
Krell InstituteUNSPECIFIED
Department of Energy (DOE)DE-FG02-97ER25308
Subject Keywords:protein folding; misfolding intermediates
PubMed Central ID:PMC4210006
Record Number:CaltechAUTHORS:20140923-113146972
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140923-113146972
Official Citation:Milo M. Lin, Dmitry Shorokhov, and Ahmed H. Zewail Dominance of misfolded intermediates in the dynamics of α-helix folding PNAS 2014 111 (40) 14424-14429; published ahead of print September 22, 2014, doi:10.1073/pnas.1416300111
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:49941
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Sep 2014 19:44
Last Modified:13 Apr 2016 21:59

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