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Table 1.
ipp-5(sy605) suppresses the sterility of
lin-3(rf) and let-23(rf) and synergizes with
other genes in the let-23-mediated fertility pathway
|
|
Genotype
|
other |
ipp-5 |
Fertile |
n |
Brood |
|
+ |
+/+ |
100% |
20 |
337 ± 33 |
let-23(rf) |
+/+ |
0% |
40 |
0 |
lin-3(rf) |
+/+ |
1.3% |
76 |
0.2 ± 1.8 |
+ |
sy605/sy605 |
100% |
25 |
144 ± 20 |
+ |
sy605/+ |
100% |
20 |
314 ± 36 |
let-23(rf) |
sy605/sy605 |
100% |
21 |
n.d.a |
lin-3(rf) |
sy605/sy605 |
98.7% |
319 |
28 ± 9 (n = 47) |
lfe-1(gf) |
+/+ |
100% |
26 |
207 ± 35 |
lfe-2(lf) |
+/+ |
100% |
23 |
213 ± 34 |
lfe-1(gf) |
sy605/sy605 |
24% |
55 |
6.0 ± 12 |
lfe-2(lf) |
sy605/sy605 |
0% |
86 |
0 |
|
Fertile is defined as having greater than five offspring. Alleles
used are lin-3(n1058), let-23(sy10),
lfe-1(sy290) and lfe-2(sy326).
unc-4(e120) was used as a marker and
mnC1[dpy-10(e128) unc-52(e444)] as a balancer for
let-23, and unc-24(e138) was used as a marker and
DnT1[nT1[unc(n745dm)let] as a balancer for lin-3.
lfe-1 was marked with unc-24(e138) and lfe-2
was marked with unc-57(ad592).
|
a
sy10 animals display a partially
penetrant larval lethal phenotype associated with let-23(rf)
decreases in RAS signaling, thus broods were not determined. n.d., not
determined.
|
|