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Flexible MEA for adult zebrafish ECG recording covering both ventricle and atrium

Zhang, Xiaoxiao and Tai, Joyce and Park, Jungwook and Tai, Yu-Chong (2014) Flexible MEA for adult zebrafish ECG recording covering both ventricle and atrium. In: 2014 IEEE 27th International Conference on Micro Electro Mechanical Systems. IEEE , Piscataway, NJ, pp. 841-844. http://resolver.caltech.edu/CaltechAUTHORS:20140930-100300402

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Abstract

This paper presents a flexible parylene micro-electrodearray (MEA) that records Electrocardiograms (ECG) from the Zebrafish heart in-vivo, covering both the ventricle and atrium area. ECG is a powerful tool for monitoring the heart activity. While ECG technology for human has been well established, this is not true for zebrafish. Our previous work demonstrated baseline ECG recording from zebrafish using MEMS MEAs [1, 2]. However, due to the body structure and small size of the zebrafish (e.g., the heart is roughly 1mm in size and its atrium is buried deep in the thoracic cavity, Fig.1b.), all zebrafish ECGs to date were only recorded from the ventricular side, making it easy to miss important electrophysiological signals from the atrium. To our knowledge, ECG from the atrial angles in Zebrafish has not yet been demonstrated. This work describes a flexible MEA implant (i.e., specially designed according to zebrafish heart anatomy) that records from both the ventricular and the atrial angles. Furthermore, to demonstrate that this device is useful for heart regeneration monitoring, our work also includes ECG recording before and after laser damage on the ventricle (532nm green light, 32mJ/mm^2, 20mJ total). This chosen energy level of laser pulse is first calibrated using ablated heart histology by EthD-1 florescence staining. The post injury ECG data clearly show ST-wave depression, an indication of ventricular abnormal repolarization state. In addition, repeated missing T-wave is observed from the channels recorded from the atrial angles, which indicate abnormalities in atrial physiology. A hypothesis is that since absorption coefficient of 532nm light in body tissue is rather low, the laser beam penetrated deeply in the heart and created damage deep in the atrium as well as the ventricle. The MEA presented here shows potential for an effective tool to study long-term adult zebrafish heart development and regeneration.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1109/MEMSYS.2014.6765772DOIArticle
http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6765772PublisherArticle
ORCID:
AuthorORCID
Tai, Yu-Chong0000-0001-8529-106X
Additional Information:© 2014 IEEE. This work is partially funded by the NIH, grant number R01 HL111437. The author would like to thank all Caltech MEMS group members for their fruitful discussions and suggestions.
Funders:
Funding AgencyGrant Number
NIHR01 HL111437
Record Number:CaltechAUTHORS:20140930-100300402
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20140930-100300402
Official Citation:Xiaoxiao Zhang; Tai, J.; Jungwook Park; Yu-chong Tai, "Flexible MEA for adult zebrafish ECG recording covering both ventricle and atrium," Micro Electro Mechanical Systems (MEMS), 2014 IEEE 27th International Conference on , vol., no., pp.841,844, 26-30 Jan. 2014 doi: 10.1109/MEMSYS.2014.6765772
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:50123
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:30 Sep 2014 21:11
Last Modified:17 May 2017 21:29

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