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Label-free electrochemical detection of human methyltransferase from tumors

Furst, Ariel L. and Muren, Natalie B. and Hill, Michael G. and Barton, Jacqueline K. (2014) Label-free electrochemical detection of human methyltransferase from tumors. Proceedings of the National Academy of Sciences of the United States of America, 111 (42). pp. 14985-14989. ISSN 0027-8424. PMCID PMC4210341. doi:10.1073/pnas.1417351111.

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The role of abnormal DNA methyltransferase activity in the development and progression of cancer is an essential and rapidly growing area of research, both for improved diagnosis and treatment. However, current technologies for the assessment of methyltransferase activity, particularly from crude tumor samples, limit this work because they rely on radioactivity or fluorescence and require bulky instrumentation. Here, we report an electrochemical platform that overcomes these limitations for the label-free detection of human DNA(cytosine-5)-methyltransferase1 (DNMT1) methyltransferase activity, enabling measurements from crude cultured colorectal cancer cell lysates (HCT116) and biopsied tumor tissues. Our multiplexed detection system involving patterning and detection from a secondary electrode array combines low-density DNA monolayer patterning and electrocatalytically amplified DNA charge transport chemistry to measure selectively and sensitively DNMT1 activity within these complex and congested cellular samples. Based on differences in DNMT1 activity measured with this assay, we distinguish colorectal tumor tissue from healthy adjacent tissue, illustrating the effectiveness of this two-electrode platform for clinical applications.

Item Type:Article
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Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2014 National Academy of Sciences. Contributed by Jacqueline K. Barton, September 8, 2014 (sent for review August 21, 2014; reviewed by Royce W. Murray). Published ahead of print October 6, 2014. We thank Dr. Bert Vogelstein (The Johns Hopkins University) for the HCT116 parent and DNMT1^(−/−) cell lines, and the National Institutes of Health (GM61077) for their support of this research. Author contributions: A.L.F., N.B.M., M.G.H., and J.K.B. designed research; A.L.F. performed research; A.L.F., N.B.M., and M.G.H. analyzed data; and A.L.F., N.B.M., and J.K.B. wrote the paper. Reviewer: R.W.M., The University of North Carolina at Chapel Hill. The authors declare no conflict of interest. This article contains supporting information online at
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Subject Keywords:DNA electrochemistry; methylation detection; electrocatalysis
Issue or Number:42
PubMed Central ID:PMC4210341
Record Number:CaltechAUTHORS:20141009-075720084
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Official Citation:Ariel L. Furst, Natalie B. Muren, Michael G. Hill, and Jacqueline K. Barton Label-free electrochemical detection of human methyltransferase from tumors PNAS 2014 111 (42) 14985-14989; published ahead of print October 6, 2014, doi:10.1073/pnas.1417351111
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:50292
Deposited By: Tony Diaz
Deposited On:09 Oct 2014 21:29
Last Modified:10 Nov 2021 18:54

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