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Effect of secondary injections of antigen upon the retention in liver of a primary injection

Garvey, Justine S. and Campbell, Dan H. (1958) Effect of secondary injections of antigen upon the retention in liver of a primary injection. Journal of Experimental Medicine, 107 (4). pp. 497-506. ISSN 0022-1007. PMCID PMC2136838. doi:10.1084/jem.107.4.497.

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The retention of antigen in rabbit liver tissue, resulting from a primary intravenous injection, is influenced by immunization brought about by subsequent intravenous injections of the same antigen. In rabbits given a single primary intravenous injection of radioactive antigen, the retention of radioactivity in liver tissue, after a period of 21 days, was greater than when the primary injection was followed by secondary injections of the same, but non-radioactive antigen. The results were similar for both S^(35)-azohemocyanin and S^(35)-azo-bovine-serum-albumin, except the hemocyanin was retained to a greater extent than the albumin. There was very little if any correlation between the number of secondary injections and retention of the initial injection. Quantitative antibody nitrogen data, obtained for the serum of each rabbit showed, in general, an inverse relationship between circulating antibody and radioactivity retained, i.e. the higher the circulating antibody titer, the lower the retention of radioactivity in liver tissue. Passively administered homologous antibody did not produce a change in the retention of the primary injection of antigen nor did secondary injections of a heterologous native protein injected according to the same immunization schedule as the homologous azoprotein. From these results it may be concluded that an intracellular antibody-forming activity influences the loss (or retention) of antigen deposited in liver tissue and that the mechanism is immunologically specific.

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Additional Information:© 1958 Rockefeller University Press. Received for publication, October 3, 1957. Submitted: 2 October 1957. This work was supported by the National Institutes of Health and The Rockefeller Foundation. The authors wish to express their appreciation of the technical assistance of Mr. Bror Clark.
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Rockefeller FoundationUNSPECIFIED
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Caltech Division of Chemistry and Chemical Engineering2252
Issue or Number:4
PubMed Central ID:PMC2136838
Record Number:CaltechAUTHORS:20141015-065108808
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Official Citation:EFFECT OF SECONDARY INJECTIONS OF ANTIGEN UPON THE RETENTION IN LIVER OF A PRIMARY INJECTION Justine S. Garvey and Dan H. Campbell J Exp Med 1958 107:497-506. Published April 1, 1958, doi:10.1084/jem.107.4.497
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:50387
Deposited By: Ruth Sustaita
Deposited On:15 Oct 2014 17:14
Last Modified:10 Nov 2021 18:55

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