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Digital, Ultrasensitive, End-Point Protein Measurements with Large Dynamic Range via Brownian Trapping with Drift

Ge, Shencheng and Liu, Weishan and Schlappi, Travis and Ismagilov, Rustem F. (2014) Digital, Ultrasensitive, End-Point Protein Measurements with Large Dynamic Range via Brownian Trapping with Drift. Journal of the American Chemical Society, 136 (42). pp. 14662-14665. ISSN 0002-7863. doi:10.1021/ja507849b. https://resolver.caltech.edu/CaltechAUTHORS:20141015-105804358

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Abstract

This communication shows that the concept of Brownian trapping with drift can be applied to improve quantitative molecular measurements. It has the potential to combine the robustness of end-point spatially resolved readouts, the ultrasensitivity of digital single-molecule measurements, and the large dynamic range of qPCR; furthermore, at low concentrations of analytes, it can provide a direct comparison of the signals arising from the analyte and from the background. It relies on the finding that molecules simultaneously diffusing, drifting (via slow flow), and binding to an array of nonsaturable surface traps have an exponentially decreasing probability of escaping the traps over time and therefore give rise to an exponentially decaying distribution of trapped molecules in space. This concept was tested with enzyme and protein measurements in a microfluidic device.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja507849bDOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja507849bPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja507849bPublisherSupporting Information
ORCID:
AuthorORCID
Schlappi, Travis0000-0001-6132-6459
Ismagilov, Rustem F.0000-0002-3680-4399
Additional Information:© 2014 American Chemical Society. ACS AuthorChoice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. Received: July 31, 2014. Publication Date (Web): October 7, 2014. This work was supported by DARPA Cooperative Agreement HR0011-11-2-0006. We thank Kevin Kan, Melissa Melendes, Shawn Hsu, Songzi Kou, Alexander Tucker-Schwartz, Mikhail Karymov, Jason Kreutz and Stephanie McCalla for discussions and experimental help, and Natasha Shelby for contributions to writing and editing this manuscript.
Funders:
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)HR0011-11-2-0006
Issue or Number:42
DOI:10.1021/ja507849b
Record Number:CaltechAUTHORS:20141015-105804358
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141015-105804358
Official Citation:Digital, Ultrasensitive, End-Point Protein Measurements with Large Dynamic Range via Brownian Trapping with Drift Shencheng Ge, Weishan Liu, Travis Schlappi, and Rustem F. Ismagilov Journal of the American Chemical Society 2014 136 (42), 14662-14665
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:50404
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Oct 2014 21:50
Last Modified:10 Nov 2021 18:55

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