Ge, Shencheng and Liu, Weishan and Schlappi, Travis and Ismagilov, Rustem F. (2014) Digital, Ultrasensitive, End-Point Protein Measurements with Large Dynamic Range via Brownian Trapping with Drift. Journal of the American Chemical Society, 136 (42). pp. 14662-14665. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20141015-105804358
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Abstract
This communication shows that the concept of Brownian trapping with drift can be applied to improve quantitative molecular measurements. It has the potential to combine the robustness of end-point spatially resolved readouts, the ultrasensitivity of digital single-molecule measurements, and the large dynamic range of qPCR; furthermore, at low concentrations of analytes, it can provide a direct comparison of the signals arising from the analyte and from the background. It relies on the finding that molecules simultaneously diffusing, drifting (via slow flow), and binding to an array of nonsaturable surface traps have an exponentially decreasing probability of escaping the traps over time and therefore give rise to an exponentially decaying distribution of trapped molecules in space. This concept was tested with enzyme and protein measurements in a microfluidic device.
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Additional Information: | © 2014 American Chemical Society. ACS AuthorChoice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. Received: July 31, 2014. Publication Date (Web): October 7, 2014. This work was supported by DARPA Cooperative Agreement HR0011-11-2-0006. We thank Kevin Kan, Melissa Melendes, Shawn Hsu, Songzi Kou, Alexander Tucker-Schwartz, Mikhail Karymov, Jason Kreutz and Stephanie McCalla for discussions and experimental help, and Natasha Shelby for contributions to writing and editing this manuscript. | ||||||||||||
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Issue or Number: | 42 | ||||||||||||
Record Number: | CaltechAUTHORS:20141015-105804358 | ||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20141015-105804358 | ||||||||||||
Official Citation: | Digital, Ultrasensitive, End-Point Protein Measurements with Large Dynamic Range via Brownian Trapping with Drift Shencheng Ge, Weishan Liu, Travis Schlappi, and Rustem F. Ismagilov Journal of the American Chemical Society 2014 136 (42), 14662-14665 | ||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
ID Code: | 50404 | ||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||
Deposited By: | Tony Diaz | ||||||||||||
Deposited On: | 16 Oct 2014 21:50 | ||||||||||||
Last Modified: | 03 Oct 2019 07:23 |
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