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Hindgut specification and cell-adhesion functions of Sphox11/13b in the endoderm of the sea urchin embryo

Arenas-Mena, César and Cameron, R. Andrew and Davidson, Eric H. (2006) Hindgut specification and cell-adhesion functions of Sphox11/13b in the endoderm of the sea urchin embryo. Development, Growth & Differentiation, 48 (7). pp. 463-472. ISSN 0012-1592. https://resolver.caltech.edu/CaltechAUTHORS:AREdgd06

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Abstract

Sphox11/13b is one of the two hox genes of Strongylocentrotus purpuratus expressed in the embryo. Its dynamic pattern of expression begins during gastrulation, when the transcripts are transiently located in a ring of cells at the edge of the blastopore. After gastrulation, expression is restricted to the anus–hindgut region at the boundary between the ectoderm and the endoderm. The phenotype that results when translation of Sphox11/13b mRNA is knocked down by treatment with morpholino antisense oligonucleotides (MASO) suggests that this gene may be indirectly involved in cell adhesion functions as well as in the proper differentiation of the midgut–hindgut and midgut–foregut sphincters. The MASO experiments also reveal that Sphox11/13b negatively regulates several downstream endomesoderm genes. For some of these genes, Sphox11/13b function is required to restrict expression to the midgut by preventing ectopic expression in the hindgut. The evolutionary conservation of these functions indicates the general roles of posterior Hox genes in regulating cell-adhesion, as well as in spatial control of gene regulatory network subcircuits in the regionalizing gut.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1111/j.1440-169X.2006.00883.xDOIUNSPECIFIED
ORCID:
AuthorORCID
Cameron, R. Andrew0000-0003-3947-6041
Additional Information:© 2006 The Authors. Journal compilation © 2006 Japanese Society of Developmental Biologists. Received 17 May 2006; revised 3 July 2006; accepted 12 July 2006. We thank Niñon Le for technical assistance and Angela Cone for thoughtful comments on the manuscript. This work was supported by the following grants: NASA/Ames NAG-1368; NSF IBN 9604454; NIH HD-37105; DOE-DE-FG02–03ER63584.
Funders:
Funding AgencyGrant Number
NASANAG-1368
NSFIBN 9604454
NIHHD-37105
Department of Energy (DOE)DE-FG02–03ER63584
Subject Keywords:cell adhesion, endoderm, hindgut, Hox, sea urchin
Issue or Number:7
Record Number:CaltechAUTHORS:AREdgd06
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:AREdgd06
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5118
Collection:CaltechAUTHORS
Deposited By: Lindsay Cleary
Deposited On:02 Oct 2006
Last Modified:02 Oct 2019 23:19

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