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Methyloprofundus sedimenti gen. nov., sp. nov., an obligate methanotroph from ocean sediment belonging to the ‘deep sea-1’ clade of marine methanotrophs

Tavormina, Patricia L. and Hatzenpichler, Roland and McGlynn, Shawn E. and Chadwick, Grayson and Dawson, Katherine S. and Connon, Stephanie A. and Orphan, Victoria J. (2015) Methyloprofundus sedimenti gen. nov., sp. nov., an obligate methanotroph from ocean sediment belonging to the ‘deep sea-1’ clade of marine methanotrophs. International Journal of Systematic and Evolutionary Microbiology, 65 (1). pp. 251-259. ISSN 1466-5026. https://resolver.caltech.edu/CaltechAUTHORS:20141111-104738855

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Abstract

We report the isolation and growth characteristics of a gammaproteobacterial methane-oxidizing bacterium (Methylococcaceae strain WF1, "whale fall 1" that shares 98% 16S rRNA identity with uncultivated free-living methanotrophs and the methanotrophic endosymbionts of deep sea mussels, 94.6% 16S rRNA identity with Methylobacter species, and 93.6% 16S rRNA identity with Methylomonas and Methylosarcina species. Strain WF1 represents the first cultivar from the 'Deep Sea 1' clade of marine methanotrophs, which includes members that participate in methane oxidation in sediments and the water column in addition to mussel endosymbionts. WF1 cells were elongated cocci approximately 1.5 µm in diameter, and occurred singly, in pairs and clumps. The cell wall was Gram negative, and stacked intracytoplasmic membranes and storage granules were evident. The genomic GC content of WF1 was 40.5%, significantly lower than currently described cultivars, and the major fatty acids were 16:0, 16:1 ω9c, 16:1 ω9t, 16:1 ω8c and 16:2 ω9, 14. Growth occurred in liquid media at an optimal temperature of 23oC, and was dependent on the presence of methane or methanol. Atmospheric nitrogen could serve as the sole nitrogen source for WF1, a capacity that had not been functionally demonstrated in members of Methylobacter. On the basis of unique morphological, physiological, and phylogenetic properties this strain represents the type species within a new genus, and we propose the name Methyloprofundus sedimenti (type strain WF1 = BCCM LMG 28393 = ATCC BAA-2619).


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1099/ijs.0.062927-0DOIArticle
http://ijs.sgmjournals.org/content/journal/ijsem/10.1099/ijs.0.062927-0PublisherArticle
ORCID:
AuthorORCID
Hatzenpichler, Roland0000-0002-5489-3444
McGlynn, Shawn E.0000-0002-8199-7011
Chadwick, Grayson0000-0003-0700-9350
Dawson, Katherine S.0000-0001-8856-4609
Orphan, Victoria J.0000-0002-5374-6178
Additional Information:© 2015 IUMS. We would like to thank Shana Goffredi, Shannon Johnson, and the captain and crew of the Western Flyer (Monterey Bay Aquarium Research Institute) for source materials for this study. We also thank Dr. Gary Martin (Occidental College) for invaluable assistance with transmission electron microscopy, and two anonymous reviewers for critical review of the work contained herein. Funding for this work was provided by grants from NASA ASTEP (NNG06GB34G, VJO) the National Science Foundation OCE (MCB-0348492, VJO), and an Erwin Schrodinger Postdoctoral Fellowship of the Austrian Science Fund (FWF), J 3162-B20 (RH).
Funders:
Funding AgencyGrant Number
NASANNG06GB34G
NSFMCB-0348492
FWF Der WissenschaftsfondsJ 3162-B20
Subject Keywords:Deep Sea 1; Methylococcaceae; methanotroph
Issue or Number:1
Record Number:CaltechAUTHORS:20141111-104738855
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141111-104738855
Official Citation:Tavormina P, Hatzenpichler R, McGlynn S, Chadwick G, Dawson K, Connon S, Orphan V. Int J Syst Evol Microbiol 65(1):251-259 doi:10.1099/ijs.0.062927-0
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:51567
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:11 Nov 2014 19:48
Last Modified:03 Oct 2019 19:22

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