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Auxin depletion from leaf primordia contributes to organ patterning

Qi, Jiyan and Wang, Ying and Yu, Ting and Cunha, Alexandre and Wu, Binbin and Vernoux, Teva and Meyerowitz, Elliot and Jiao, Yuling (2014) Auxin depletion from leaf primordia contributes to organ patterning. Proceedings of the National Academy of Sciences of the United States of America, 111 (52). pp. 18769-18774. ISSN 0027-8424. PMCID PMC4284573. https://resolver.caltech.edu/CaltechAUTHORS:20141111-165343277

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Abstract

Stem cells are responsible for organogenesis, but it is largely unknown whether and how information from stem cells acts to direct organ patterning after organ primordia are formed. It has long been proposed that the stem cells at the plant shoot apex produce a signal, which promotes leaf adaxial-abaxial (dorsoventral) patterning. Here we show the existence of a transient low auxin zone in the adaxial domain of early leaf primordia. We also demonstrate that this adaxial low auxin domain contributes to leaf adaxial-abaxial patterning. The auxin signal is mediated by the auxin-responsive transcription factor MONOPTEROS (MP), whose constitutive activation in the adaxial domain promotes abaxial cell fate. Furthermore, we show that auxin flow from emerging leaf primordia to the shoot apical meristem establishes the low auxin zone, and that this auxin flow contributes to leaf polarity. Our results provide an explanation for the hypothetical meristem-derived leaf polarity signal. Opposite to the original proposal, instead of a signal derived from the meristem, we show that a signaling molecule is departing from the primordium to the meristem to promote robustness in leaf patterning.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.1421878112 DOIArticle
http://www.pnas.org/content/111/52/18769.abstractPublisherArticle
http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1421878112/-/DCSupplementalPublisherSupporting Information
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284573/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot0000-0003-4798-5153
Jiao, Yuling0000-0002-1189-1676
Additional Information:Copyright © 2014 National Academy of Sciences. Contributed by Elliot Meyerowitz, November 20, 2014 (sent for review August 14, 2014; reviewed by Yuval Eshed and Lars Østergaard) Published online before print December 15, 2014, doi: 10.1073/pnas.1421878112 We thank Drs. N. Ori, E. A. Schultz, and D. Weijers for seeds; Dr. C. Li for allowing us to use his tomato growth facility; and Drs. Y. Eshed, H. Huang, C. Kuhlemeier, O. Leyser, and L. Xu for discussions. Y.J. thanks Profs. S.-N. Bai and Z.-H. Xu for their encouragement. This work was supported by National Basic Research Program of China (973 Program) Grant 2014CB943500, National Natural Science Foundation of China Grant 31222033, State Key Laboratory of Plant Genomics Grant SKLPG2011B0103, US National Science Foundation 2010 Project Grant MCB-0929349, and France ANR-12-BSV6-0005 Grant (AuxiFlo). The E.M. laboratory is also supported by funds from the Howard Hughes Medical Institute and Gordon and Betty Moore Foundation Grant GBMF3406, and the Y.J. laboratory is supported by the Hundred Talents Program of the Chinese Academy of Sciences. J.Q. and Y.W. contributed equally to this work. Author contributions: J.Q., Y.W., T.Y., B.W., and T.V. performed research; Y.W., E.M., and Y.J. designed research; A.C. and T.V. contributed new reagents/analytic tools; J.Q., Y.W., A.C., T.V., E.M., and Y.J. analyzed data; and T.V., E.M., and Y.J. wrote the paper. Reviewers: Y.E., Weizmann Institute of Science; and L.Ø., John Innes Centre. The authors declare no conflict of interest. This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1421878112/-/DCSupplemental.
Funders:
Funding AgencyGrant Number
National Basic Research Program of China (973 Program)2014CB943500
National Natural Science Foundation of China31222033
State Key Laboratory of Plant GenomicsSKLPG2011B0103
NSFMCB-0929349
Agence Nationale de la Recherche (ANR)ANR-12-BSV6-0005
Subject Keywords:auxin; leaf polarity; meristem; stem cell
Issue or Number:52
PubMed Central ID:PMC4284573
Record Number:CaltechAUTHORS:20141111-165343277
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141111-165343277
Official Citation:Jiyan Qi, Ying Wang, Ting Yu, Alexandre Cunha, Binbin Wu, Teva Vernoux, Elliot Meyerowitz, and Yuling Jiao Auxin depletion from leaf primordia contributes to organ patterning PNAS 2014 111 (52) 18769-18774; published ahead of print December 15, 2014, doi:10.1073/pnas.1421878112
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:51621
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:17 Dec 2014 22:54
Last Modified:03 Oct 2019 07:34

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