Debler, Erik W. and Kaufmann, Gunnar F. and Meijler, Michael M. and Heine, Andreas and Mee, Jenny M. and Pljevaljčić, Goran and Di Bilio, Angel J. and Schultz, Peter G. and Millar, David P. and Janda, Kim D. and Wilson, Ian A. and Gray, Harry B. and Lerner, Richard A. (2008) Deeply Inverted Electron-Hole Recombination in a Luminescent Antibody-Stilbene Complex. Science, 319 (5867). pp. 1232-1235. ISSN 0036-8075. doi:10.1126/science.1153445. https://resolver.caltech.edu/CaltechAUTHORS:20141114-093033379
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Abstract
The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel π-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.
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| Additional Information: | © 2008 American Association for the Advancement of Science. Received 27 November 2007; accepted 18 January 2008. We thank S. Ferguson for assistance in Fab preparation and the staff at Advanced Light Source beamline 5.0.1 for their support. We gratefully acknowledge A. Deniz for providing access to his fluorimeter, and S. Mukhopadhyay for assistance with anisotropy measurements as well as helpful discussions. We thank J. Saltiel as well as the reviewers for critically reading the manuscript and providing helpful comments. Coordinates and structure factors for the EP2-25C10-1 complex have been deposited at the Protein Data Bank (PDB) with accession number 2NZR. Supported by NIH grants GM38273 (I.A.W.), GM56528 (P.G.S.), and DK19038 (H.B.G.) and a Skaggs predoctoral fellowship and Jairo H. Arévalo fellowship from The Scripps Research Institute graduate program (E.W.D.). This is publication 18959-MB from The Scripps Research Institute. | ||||||||||||
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| Issue or Number: | 5867 | ||||||||||||
| DOI: | 10.1126/science.1153445 | ||||||||||||
| Record Number: | CaltechAUTHORS:20141114-093033379 | ||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20141114-093033379 | ||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
| ID Code: | 51753 | ||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||
| Deposited By: | Tony Diaz | ||||||||||||
| Deposited On: | 14 Nov 2014 22:18 | ||||||||||||
| Last Modified: | 10 Nov 2021 19:15 |
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