Lois, Carlos and Hong, Elizabeth J. and Pease, Shirley and Brown, Eric J. and Baltimore, David (2002) Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral Vectors. Science, 295 (5556). pp. 868-872. ISSN 0036-8075. doi:10.1126/science.1067081. https://resolver.caltech.edu/CaltechAUTHORS:20141117-132359069
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Abstract
Single-cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter. Eighty percent of founder mice carried at least one copy of the transgene, and 90% of these expressed GFP at high levels. Progeny inherited the transgene(s) and displayed green fluorescence. Mice generated using lentiviral vectors with muscle-specific and T lymphocyte–specific promoters expressed high levels of GFP only in the appropriate cell types. We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species.
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Additional Information: | © 2002 American Association for the Advancement of Science. Received 12 October 2001; accepted 27 December 2001; Published online 10 January 2002. We thank I. Verma, D. Trono, L. Theil, and H. Wong for providing reagents; X. F. Qin and M. Laurent for help with the FACS analysis; and B. Wold for useful discussions. Supported by grants from the National Institutes of Health (D.B.). C.L. is supported by a Christopher Reeves Paralysis Foundation fellowship. | ||||||||||||
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Issue or Number: | 5556 | ||||||||||||
DOI: | 10.1126/science.1067081 | ||||||||||||
Record Number: | CaltechAUTHORS:20141117-132359069 | ||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20141117-132359069 | ||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
ID Code: | 51854 | ||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||
Deposited By: | Tony Diaz | ||||||||||||
Deposited On: | 17 Nov 2014 21:33 | ||||||||||||
Last Modified: | 10 Nov 2021 19:16 |
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