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Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral Vectors

Lois, Carlos and Hong, Elizabeth J. and Pease, Shirley and Brown, Eric J. and Baltimore, David (2002) Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral Vectors. Science, 295 (5556). pp. 868-872. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20141117-132359069

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Abstract

Single-cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter. Eighty percent of founder mice carried at least one copy of the transgene, and 90% of these expressed GFP at high levels. Progeny inherited the transgene(s) and displayed green fluorescence. Mice generated using lentiviral vectors with muscle-specific and T lymphocyte–specific promoters expressed high levels of GFP only in the appropriate cell types. We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.1067081DOIArticle
http://www.sciencemag.org/content/295/5556/868PublisherArticle
http://www.sciencemag.org/content/295/5556/868/suppl/DC1PublisherSupplemental Data
ORCID:
AuthorORCID
Lois, Carlos0000-0002-7305-2317
Baltimore, David0000-0001-8723-8190
Additional Information:© 2002 American Association for the Advancement of Science. Received 12 October 2001; accepted 27 December 2001; Published online 10 January 2002. We thank I. Verma, D. Trono, L. Theil, and H. Wong for providing reagents; X. F. Qin and M. Laurent for help with the FACS analysis; and B. Wold for useful discussions. Supported by grants from the National Institutes of Health (D.B.). C.L. is supported by a Christopher Reeves Paralysis Foundation fellowship.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
Christopher Reeves Paralysis FoundationUNSPECIFIED
Issue or Number:5556
Record Number:CaltechAUTHORS:20141117-132359069
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141117-132359069
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:51854
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:17 Nov 2014 21:33
Last Modified:03 Oct 2019 07:36

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