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Promotion of NEDD8-CUL1 Conjugate Cleavage by COP9 Signalosome

Lyapina, Svetlana and Cope, Gregory and Shevchenko, Anna and Serino, Giovanna and Tsuge, Tomohiko and Zhou, Chunshui and Wolf, Dieter A. and Wei, Ning and Shevchenko, Andrej and Deshaies, Raymond J. (2001) Promotion of NEDD8-CUL1 Conjugate Cleavage by COP9 Signalosome. Science, 292 (5520). pp. 1382-1385. ISSN 0036-8075.

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SCF ubiquitin ligases control various processes by marking regulatory proteins for ubiquitin-dependent proteolysis. To illuminate how SCF complexes are regulated, we sought proteins that interact with the human SCF component CUL1. The COP9 signalosome (CSN), a suppressor of plant photomorphogenesis, associated with multiple cullins and promoted cleavage of the ubiquitin-like protein NEDD8 from Schizosaccharomyces pombe CUL1 in vivo and in vitro. Multiple NEDD8-modified proteins uniquely accumulated in CSN-deficient S. pombe cells. We propose that the broad spectrum of activities previously attributed to CSN subunits—including repression of photomorphogenesis, activation of JUN, and activation of p27 nuclear export—underscores the importance of dynamic cycles of NEDD8 attachment and removal in biological regulation.

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Deshaies, Raymond J.0000-0002-3671-9354
Additional Information:© 2001 American Association for the Advancement of Science. 12 February 2001; accepted 17 April 2001. Published Online May 3 2001. We thank C. Lois, J Roberts, T. Caspari, A. Carr, S. Forsburg, J. Thorner, T. Toda, F. Osaka, Y. Xiong, and P. Nurse for generously providing retroviral vectors, S. pombe strains, expression plasmids, and antibodies; M. Petroski for 32P-labeled ubiquitin; and R. Verma for experimental input, helpful discussions, and pointing out JAB1 homology to ubiquitin-specific proteases. We also thank P. Jackson and members of his laboratory for hosting this project in its early days. Supported by an Amgen fellowship (S.L.) and by the W. M. Keck Foundation and HHMI.
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W. M. Keck FoundationUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:5520
Record Number:CaltechAUTHORS:20141118-095512539
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Official Citation:Promotion of NEDD8-CUL1 Conjugate Cleavage by COP9 Signalosome Svetlana Lyapina, Gregory Cope, Anna Shevchenko, Giovanna Serino, Tomohiko Tsuge, Chunshui Zhou, Dieter A. Wolf, Ning Wei, Andrej Shevchenko, and Raymond J. Deshaies Science 18 May 2001: 292 (5520), 1382-1385.Published online 3 May 2001 [DOI:10.1126/science.1059780]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:51893
Deposited By: Ruth Sustaita
Deposited On:18 Nov 2014 18:25
Last Modified:03 Oct 2019 07:37

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