CaltechAUTHORS
  A Caltech Library Service

Discovery of a Major D-Loop Replication Origin Reveals Two Modes of Human mtDNA Synthesis

Fish, Jennifer and Raule, Nicola and Attardi, Giuseppe (2004) Discovery of a Major D-Loop Replication Origin Reveals Two Modes of Human mtDNA Synthesis. Science, 306 (5704). pp. 2098-2101. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20141118-131413243

[img]
Preview
PDF (Materials and Methods; SOM Text; Figs. S1 to S7'; References) - Supplemental Material
See Usage Policy.

1015Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20141118-131413243

Abstract

Mammalian mitochondrial DNA (mtDNA) replication has long been considered to occur by asymmetric synthesis of the two strands, starting at the multiple origins of the strand-displacement loop (D-loop). We report the discovery of a major replication origin at position 57 in the D-loop of several human cell lines (HeLa, A549, and 143B.TK^–) and immortalized lymphocytes. The nascent chains starting at this origin, in contrast to those initiated at the previously described origins, do not terminate prematurely at the 3′ end of the D-loop but proceed well beyond this control point, behaving as “true” replicating strands. This origin is mainly responsible for mtDNA maintenance under steady-state conditions, whereas mtDNA synthesis from the formerly identified D-loop origins may be more important for recovery after mtDNA depletion and for accelerating mtDNA replication in response to physiological demands.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.1102077 DOIArticle
http://www.sciencemag.org/content/306/5704/2098PublisherArticle
http://www.sciencemag.org/content/306/5704/2098/suppl/DC1PublisherSupporting Online Material
Additional Information:© 2004 American Association for the Advancement of Science. 28 June 2004; accepted 21 October 2004. Supported by NIH grant GM11726 (G.A.). We thank J. Zhang for providing additional data, A. Chomyn for valuable discussions and comments on the manuscript, and M. Roldan-Ortiz and R. Zedan for expert technical assistance.
Funders:
Funding AgencyGrant Number
NIHGM11726
Issue or Number:5704
Record Number:CaltechAUTHORS:20141118-131413243
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141118-131413243
Official Citation:Fish, J., Raule, N., & Attardi, G. (2004). Discovery of a Major D-Loop Replication Origin Reveals Two Modes of Human mtDNA Synthesis. Science, 306(5704), 2098-2101. doi: 10.1126/science.1102077
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:51909
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:18 Nov 2014 23:55
Last Modified:03 Oct 2019 07:37

Repository Staff Only: item control page