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Cell Dynamics During Somite Boundary Formation Revealed by Time-Lapse Analysis

Kulesa, Paul M. and Fraser, Scott E. (2002) Cell Dynamics During Somite Boundary Formation Revealed by Time-Lapse Analysis. Science, 298 (5595). pp. 991-995. ISSN 0036-8075. doi:10.1126/science.1075544. https://resolver.caltech.edu/CaltechAUTHORS:20141119-094303997

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Abstract

We follow somite segmentation in living chick embryos and find that the shaping process is not a simple periodic slicing of tissue blocks but a much more carefully choreographed separation in which the somite pulls apart from the segmental plate. Cells move across the presumptive somite boundary and violate gene expression boundaries thought to correlate with the site of the somite boundary. Similarly, cells do not appear to be preassigned to a given somite as they leave the node. The results offer a detailed picture of somite shaping and provide a spatiotemporal framework for linking gene expression with cell movements.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.sciencemag.org/content/298/5595/991.abstractPublisherArticle
http://dx.doi.org/10.1126/science.1075544 DOIArticle
http://www.sciencemag.org/content/298/5595/991/suppl/DC1Related ItemSupplementary Material
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2002 American Association for the Advancement of Science. 27 June 2002; accepted28 August 2002. We thank M. Dickinson andJ. Kastner for comments on the manuscript andhelp with in situ hybridizations, and A. Ewald, H. McBride, M. Reddington, and R. Kerschman for their help with the serial sectioning by surface imaging microscopy (30). P.M.K. is a participant in the California Institute of Technology Initiative in Computational Molecular Biology, which is funded by a Burroughs Wellcome Fund Interfaces award.
Funders:
Funding AgencyGrant Number
Burroughs Wellcome Fund Interfaces AwardUNSPECIFIED
Issue or Number:5595
DOI:10.1126/science.1075544
Record Number:CaltechAUTHORS:20141119-094303997
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141119-094303997
Official Citation:Cell Dynamics During Somite Boundary Formation Revealed by Time-Lapse Analysis Paul M. Kulesa and Scott E. Fraser Science 1 November 2002: 298 (5595), 991-995. [DOI:10.1126/science.1075544]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:51948
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:19 Nov 2014 21:28
Last Modified:10 Nov 2021 19:18

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