Steger, David J. and Haswell, Elizabeth S. and Miller, Aimee L. and Wente, Susan R. and O'Shea, Erin K. (2003) Regulation of Chromatin Remodeling by Inositol Polyphosphates. Science, 299 (5603). pp. 114-116. ISSN 0036-8075. doi:10.1126/science.1078062. https://resolver.caltech.edu/CaltechAUTHORS:20141119-121533180
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Abstract
Chromatin remodeling is required for efficient transcription of eukaryotic genes. In a genetic selection for budding yeast mutants that were defective in induction of the phosphate-responsive PHO5 gene, we identified mutations inARG82/IPK2, which encodes a nuclear inositol polyphosphate kinase. In arg82 mutant strains, remodeling ofPHO5 promoter chromatin is impaired, and the adenosine triphosphate–dependent chromatin-remodeling complexes SWI/SNF and INO80 are not efficiently recruited to phosphate-responsive promoters. These results suggest a role for the small molecule inositol polyphosphate in the regulation of chromatin remodeling and transcription.
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| Additional Information: | © 2002 American Association for the Advancement of Science. 4 September 2002; accepted 31 October 2002. Published online 14 November 2002. We thank C. Wu for communicating results before publication, F. Messenguy and J. York for ARG82 plasmids, and members of the O’Shea laboratory and T. Gruber for helpful discussions. Supported by NIH (GM51377 to E.K.O. and GM51219 to S.R.W.), the Steven and Michelle Kirsch Foundation (S.R.W.), the Howard Hughes Medical Institute (E.K.O.), and the David and Lucile Packard Foundation (E.K.O.). D.J.S. is a Leukemia and Lymphoma Society Special Fellow. | ||||||||||||||
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| Issue or Number: | 5603 | ||||||||||||||
| DOI: | 10.1126/science.1078062 | ||||||||||||||
| Record Number: | CaltechAUTHORS:20141119-121533180 | ||||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20141119-121533180 | ||||||||||||||
| Official Citation: | Regulation of Chromatin Remodeling by Inositol Polyphosphates David J. Steger, Elizabeth S. Haswell, Aimee L. Miller, Susan R. Wente, and Erin K. O'Shea Science 3 January 2003: 299 (5603), 114-116. Published online 14 November 2002 [DOI:10.1126/science.1078062] | ||||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||||
| ID Code: | 51967 | ||||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||||
| Deposited By: | Ruth Sustaita | ||||||||||||||
| Deposited On: | 19 Nov 2014 23:15 | ||||||||||||||
| Last Modified: | 02 Mar 2023 22:33 |
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