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Dynamic association of transcriptional activation domains and regulatory regions in Saccharomyces cerevisiae heat shock factor

Chen, Tianxin and Parker, Carl S. (2002) Dynamic association of transcriptional activation domains and regulatory regions in Saccharomyces cerevisiae heat shock factor. Proceedings of the National Academy of Sciences of the United States of America, 99 (3). pp. 1200-1205. ISSN 0027-8424. PMCID PMC122167. doi:10.1073/pnas.032681299. https://resolver.caltech.edu/CaltechAUTHORS:20141119-131958366

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Abstract

In Saccharomyces cerevisiae, the heat shock transcription factor (HSF) is thought to be a homotypic trimer that is bound to the promoters of heat shock protein (HSP) genes at both normal and heat shock temperatures. Exposure to heat shock greatly and rapidly induces HSF transcriptional activity without further increasing DNA-binding affinity. It is believed that HSF is under negative regulation at normal growth temperatures, but the detailed mechanism by which HSF is activated is still not clear. We report the analysis of mutations in a conserved arginine (residue 274) at the C-terminal end of the DNA-binding domain (DBD). Two mutations significantly increase both basal activity of HSF at normal temperatures and induced activity on heat shock. We demonstrate by coimmunoprecipitation experiments that the mutations reduce the association between the DNA-binding domain/oligomerization domain and the transcription activation domains. Our studies suggest that the DNA-binding domain of HSF can interact with activation domains directly, and this interaction is important for the repression of HSF activity under normal growth conditions. Destabilizing this interaction by heat or by mutations results in HSF transcriptional activation. We propose that Arg-274 is critical for intramolecular repression of HSF activity in normally growing cells.


Item Type:Article
Related URLs:
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http://dx.doi.org/10.1073/pnas.032681299DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc122167/PubMed CentralArticle
Additional Information:© 2002 National Academy of Sciences. Communicated by Douglas C. Rees, California Institute of Technology, Pasadena, CA, December 18, 2001 (received for review June 12, 2001). Published ahead of print January 29, 2002. We thank Dr. Xiangdong Fang for suggestions on protein purification. We thank Dr. Duncan Odom for helpful comments on the manuscript. This work was supported by National Institutes of Health Grant GM47381 (to C.S.P.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHGM47381
Issue or Number:3
PubMed Central ID:PMC122167
DOI:10.1073/pnas.032681299
Record Number:CaltechAUTHORS:20141119-131958366
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141119-131958366
Official Citation:Chen, T., & Parker, C. S. (2002). Dynamic association of transcriptional activation domains and regulatory regions in Saccharomyces cerevisiae heat shock factor. Proceedings of the National Academy of Sciences, 99(3), 1200-1205. doi: 10.1073/pnas.032681299
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:51972
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:19 Nov 2014 22:59
Last Modified:10 Nov 2021 19:18

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