CaltechAUTHORS
  A Caltech Library Service

Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana

Chuang, Chiou-Fen and Meyerowitz, Elliot M. (2000) Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America, 97 (9). pp. 4985-4990. ISSN 0027-8424. PMCID PMC18344. http://resolver.caltech.edu/CaltechAUTHORS:20141119-140957534

[img]
Preview
PDF - Published Version
See Usage Policy.

598Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20141119-140957534

Abstract

We investigated the potential of double-stranded RNA interference (RNAi) with gene activity in Arabidopsis thaliana. To construct transformation vectors that produce RNAs capable of duplex formation, gene-specific sequences in the sense and antisense orientations were linked and placed under the control of a strong viral promoter. When introduced into the genome of A. thaliana by Agrobacterium-mediated transformation, double-stranded RNA-expressing constructs corresponding to four genes, AGAMOUS (AG), CLAVATA3, APETALA1, and PERIANTHIA, caused specific and heritable genetic interference. The severity of phenotypes varied between transgenic lines. In situ hybridization revealed a correlation between a declining AG mRNA accumulation and increasingly severe phenotypes in AG (RNAi) mutants, suggesting that endogenous mRNA is the target of double-stranded RNA-mediated genetic interference. The ability to generate stably heritable RNAi and the resultant specific phenotypes allows us to selectively reduce gene function in A. thaliana.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.060034297 DOIArticle
http://www.pnas.org/content/97/9/4985PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC18344/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2000 National Academy of Sciences. Contributed by Elliot M. Meyerowitz, January 24, 2000. Published online before print April 18, 2000. We thank John Bowman and Yuval Eshed for critical reading of the manuscript; Xavier Ambroggio, Catherine Baker, Chieh Chang, Toshiro Ito, Jeff Long, Kazuaki Ohashi, Carolyn Ohno, G. Venugopala Reddy, Doris Wagner, Frank Wellmer, and Eva Ziegelhoffer for their comments on the manuscript; Amani Zewail for technical assistance; and Toshiro Ito for providing the anti-AG antibody. This work was supported by National Institutes of Health Grant GM45697 to E.M.M.
Funders:
Funding AgencyGrant Number
NIHGM45697
PubMed Central ID:PMC18344
Record Number:CaltechAUTHORS:20141119-140957534
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20141119-140957534
Official Citation:Chiou-Fen Chuang and Elliot M. Meyerowitz Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana PNAS 2000 97 (9) 4985-4990; published ahead of print April 18, 2000, doi:10.1073/pnas.060034297
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:51975
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 Nov 2014 23:26
Last Modified:20 Jul 2017 19:56

Repository Staff Only: item control page