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Cloning of the Arabidopsis WIGGUM gene identifies a role for farnesylation in meristem development

Ziegelhoffer, Eva C. and Medrano, Leonard J. and Meyerowitz, Elliot M. (2000) Cloning of the Arabidopsis WIGGUM gene identifies a role for farnesylation in meristem development. Proceedings of the National Academy of Sciences of the United States of America, 97 (13). pp. 7633-7638. ISSN 0027-8424. PMCID PMC16597. http://resolver.caltech.edu/CaltechAUTHORS:20141119-145257875

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Abstract

Control of cellular proliferation in plant meristems is important for maintaining the correct number and position of developing organs. One of the genes identified in the control of floral and apical meristem size and floral organ number in Arabidopsis thaliana is WIGGUM. In wiggum mutants, one of the most striking phenotypes is an increase in floral organ number, particularly in the sepals and petals, correlating with an increase in the width of young floral meristems. Additional phenotypes include reduced and delayed germination, delayed flowering, maturation, and senescence, decreased internode elongation, shortened roots, aberrant phyllotaxy of flowers, aberrant sepal development, floral buds that open precociously, and occasional apical meristem fasciation. As a first step in determining a molecular function for WIGGUM, we used positional cloning to identify the gene. DNA sequencing revealed that WIGGUM is identical to ERA1 (enhanced response to abscisic acid), a previously identified farnesyltransferase β-subunit gene of Arabidopsis. This finding provides a link between protein modification by farnesylation and the control of meristem size. Using in situ hybridization, we examined the expression of ERA1 throughout development and found it to be nearly ubiquitous. This extensive expression domain is consistent with the pleiotropic nature of wiggum mutants and highlights a broad utility for farnesylation in plant growth and development.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.130189397 DOIArticle
http://www.pnas.org/content/97/13/7633PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC16597/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2000 National Academy of Sciences. Contributed by Elliot M. Meyerowitz, April 26, 2000. Published ahead of print June 6, 2000. We thank Mark Running, Jenn Fletcher, and Jeff Long for technical advice, John Larkin and Jason Walker for sharing mapping data and CAPS markers, the Arabidopsis Biological Resource Center and the Kazusa DNA Research Institute for the MSN9 clone, and members of the Meyerowitz lab for comments on the manuscript. This work was supported by National Science Foundation Grant MCB9603821 (to E.M.M.). E.C.Z. was supported by a Caltech Biology Division Gosney Fellowship and National Institutes of Health Postdoctoral Fellowship GM19500. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NSFMCB9603821
Caltech Gosney FellowshipUNSPECIFIED
NIHGM19500
PubMed Central ID:PMC16597
Record Number:CaltechAUTHORS:20141119-145257875
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20141119-145257875
Official Citation:Eva C. Ziegelhoffer, Leonard J. Medrano, and Elliot M. Meyerowitz Cloning of the Arabidopsis WIGGUM gene identifies a role for farnesylation in meristem development PNAS 2000 97 (13) 7633-7638; published ahead of print June 6, 2000, doi:10.1073/pnas.130189397
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:51978
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 Nov 2014 23:24
Last Modified:20 Jul 2017 19:50

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