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Physiologically relevant and portable tandem ubiquitin-binding domain stabilizes polyubiquitylated proteins

Tyrrell, An and Flick, Karin and Kleiger, Gary and Zhang, Hongwei and Deshaies, Raymond J. and Kaiser, Peter (2010) Physiologically relevant and portable tandem ubiquitin-binding domain stabilizes polyubiquitylated proteins. Proceedings of the National Academy of Sciences of the United States of America, 107 (46). pp. 19796-19801. ISSN 0027-8424. PMCID PMC2993400. https://resolver.caltech.edu/CaltechAUTHORS:20141120-133841734

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Abstract

Ubiquitylation of proteins can be a signal for a variety of cellular processes beyond the classical role in proteolysis. The different signaling functions of ubiquitylation are thought to rely on ubiquitin-binding domains (UBDs). Several distinct UBD families are known, but their functions are not understood in detail, and mechanisms for interpretation and transmission of the ubiquitin signals remain to be discovered. One interesting example of the complexity of ubiquitin signaling is the Saccharomyces cerevisiae transcription factor Met4, which is regulated by a single lysine-48 linked polyubiquitin chain that can directly repress activity of Met4 or induce degradation by the proteasome. Here we show that ubiquitin signaling in Met4 is controlled by its tandem UBD regions, consisting of a previously recognized ubiquitin-interacting motif and a novel ubiquitin-binding region, which lacks homology to known UBDs. The tandem arrangement of UBDs is required to protect ubiquitylated Met4 from degradation and enables direct inactivation of Met4 by ubiquitylation. Interestingly, protection from proteasomes is a portable feature of UBDs because a fusion of the tandem UBDs to the classic proteasome substrate Sic1 stabilized Sic1 in vivo in its ubiquitylated form. Using the well-defined Sic1 in vitro ubiquitylation system we demonstrate that the tandem UBDs inhibit efficient polyubiquitin chain elongation but have no effect on initiation of ubiquitylation. Importantly, we show that the nonproteolytic regulation enabled by the tandem UBDs is critical for ensuring rapid transcriptional responses to nutritional stress, thus demonstrating an important physiological function for tandem ubiquitin-binding domains that protect ubiquitylated proteins from degradation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.1010648107 DOIArticle
http://www.pnas.org/content/107/46/19796PublisherArticle
http://www.pnas.org/content/107/46/19796?tab=dsPublisherSupporting Information
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2993400/PubMed CentralArticle
ORCID:
AuthorORCID
Kleiger, Gary0000-0003-3924-1680
Deshaies, Raymond J.0000-0002-3671-9354
Additional Information:© 2010 National Academy of Sciences. Edited by Alexander Varshavsky, California Institute of Technology, Pasadena, CA, and approved September 29, 2010 (received for review July 22, 2010). Published ahead of print November 1, 2010. We thank N. Dantuma and C. Heinen for discussion of unpublished data. This work was supported by National Institutes of Health Grants GM66164 and GM66164AS1 (P.K.) and A.T. is supported by an NCI Institutional Training Grant Award (5 T32 CA009054-32). R.J.D. is an Investigator of the Howard Hughes Medical Institute. Author contributions: A.T., K.F., G.K., R.J.D., and P.K. designed research; A.T., K.F., G.K., H.Z., and P.K. performed research; G.K., H.Z., and R.J.D. contributed new reagents/analytic tools; A.T., K.F., G.K., H.Z., R.J.D., and P.K. analyzed data; and A.T., K.F., and P.K. wrote the paper. The authors declare no conflict of interest.
Funders:
Funding AgencyGrant Number
NIHGM66164
NIHGM66164AS1
NCI Institutional Training Grant Award5 T32 CA009054-32
Subject Keywords:protein degradation; polyubiquitin chain protection
Issue or Number:46
PubMed Central ID:PMC2993400
Record Number:CaltechAUTHORS:20141120-133841734
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141120-133841734
Official Citation:An Tyrrell, Karin Flick, Gary Kleiger, Hongwei Zhang, Raymond J. Deshaies, and Peter Kaiser Physiologically relevant and portable tandem ubiquitin-binding domain stabilizes polyubiquitylated proteins PNAS 2010 107 (46) 19796-19801; published ahead of print November 1, 2010, doi:10.1073/pnas.1010648107
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52010
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:20 Nov 2014 22:57
Last Modified:09 Mar 2020 13:18

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