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Engineering Cooperativity in Biomotor-Protein Assemblies

Diehl, Michael R. and Zhang, Kechun and Lee, Heun Jin and Tirrell, David A. (2006) Engineering Cooperativity in Biomotor-Protein Assemblies. Science, 311 (5766). pp. 1468-1471. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20141120-161852368

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Abstract

A biosynthetic approach was developed to control and probe cooperativity in multiunit biomotor assemblies by linking molecular motors to artificial protein scaffolds. This approach provides precise control over spatial and elastic coupling between motors. Cooperative interactions between monomeric kinesin-1 motors attached to protein scaffolds enhance hydrolysis activity and microtubule gliding velocity. However, these interactions are not influenced by changes in the elastic properties of the scaffold, distinguishing multimotor transport from that powered by unorganized monomeric motors. These results highlight the role of supramolecular architecture in determining mechanisms of collective transport.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.1122125 DOIArticle
http://www.sciencemag.org/content/311/5766/1468PublisherArticle
http://www.sciencemag.org/content/311/5766/1468/suppl/DC1PublisherSupporting Information
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 2006 American Association for the Advancement of Science. 2 November 2005; Accepted 6 February 2006. We thank A. J. Link and I. Fushman their help during the early stages of this project; L. Wade and D. Pearson for use of the temperature controller; and P. Wiggins, R. Bao, T. Squires, and S. Quake for valuable discussions. This work was supported by the Beckman Foundation through a Beckman Senior Research Fellowship (to M.R.D.) and by a grant from the National Science Foundation.
Funders:
Funding AgencyGrant Number
Arnold and Mabel Beckman FoundationUNSPECIFIED
NSFUNSPECIFIED
Record Number:CaltechAUTHORS:20141120-161852368
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20141120-161852368
Official Citation:Diehl, M. R., Zhang, K., Lee, H. J., & Tirrell, D. A. (2006). Engineering Cooperativity in Biomotor-Protein Assemblies. Science, 311(5766), 1468-1471. doi: 10.1126/science.1122125
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52025
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:21 Nov 2014 17:34
Last Modified:15 Jan 2015 19:24

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