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Long-Term Monitoring of Bacteria Undergoing Programmed Population Control in a Microchemostat

Balagaddé, Frederick K. and You, Lingchong and Hansen, Carl L. and Arnold, Frances H. and Quake, Stephen R. (2005) Long-Term Monitoring of Bacteria Undergoing Programmed Population Control in a Microchemostat. Science, 309 (5731). pp. 137-140. ISSN 0036-8075. doi:10.1126/science.1109173.

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Using an active approach to preventing biofilm formation, we implemented a microfluidic bioreactor that enables long-term culture and monitoring of extremely small populations of bacteria with single-cell resolution. We used this device to observe the dynamics of Escherichia coli carrying a synthetic “population control” circuit that regulates cell density through a feedback mechanism based on quorum sensing. The microfluidic bioreactor enabled long-term monitoring of unnatural behavior programmed by the synthetic circuit, which included sustained oscillations in cell density and associated morphological changes, over hundreds of hours.

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Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2005 American Association for the Advancement of Science. Received 27 December 2004; accepted 10 May 2005. We thank M. Elowitz for MC4100Z1 cells, U. Alon for MG1655 cells, T. Ozdere for generating data for figs. S4 and S5, C. Collins for plasmid pLuxR, M. Barnett for technical assistance, and J. Leadbetter, C. Ward, T. Squires, J. Huang, and E. Kartalov for helpful discussions. Supported in part by the NSF and the Defense Advanced Research Projects Agency (contract no. N66001-02-1-8929).
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Defense Advanced Research Projects Agency (DARPA)N66001-02-1-8929
Issue or Number:5731
Record Number:CaltechAUTHORS:20141121-103618066
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52041
Deposited By: Tony Diaz
Deposited On:21 Nov 2014 20:45
Last Modified:10 Nov 2021 19:19

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