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Shizuya, Hiroaki and Birren, Bruce and Kim, Ung-Jin and Mancino, Valeria and Slepak, Tatiana and Tachiiri, Yoshiaki and Simon, Melvin (1992) . Proceedings of the National Academy of Sciences of the United States of America, 89 (18). pp. 8794-8797. ISSN 0027-8424. PMCID PMC50007. doi:10.1073/pnas.89.18.8794.

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A bacterial cloning system for mapping and analysis of complex genomes has been developed. The BAC system (for bacterial artificial chromosome) is based on Escherichia coli and its single-copy plasmid F factor. It is capable of maintaining human genomic DNA fragments of greater than 300 kilobase pairs. Individual clones of human DNA appear to be maintained with a high degree of structural stability in the host, even after 100 generations of serial growth. Because of high cloning efficiency, easy manipulation of the cloned DNA, and stable maintenance of inserted DNA, the BAC system may facilitate construction of DNA libraries of complex genomes with fuller representation and subsequent rapid analysis of complex genomic structure.

Item Type:Article
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Additional Information:© 1992 National Academy of Sciences. Contributed by Melvin Simon, June 30, 1992. We thank Drs. M. O'Connor and W. Bender for providing the pMBO131 plasmid prior to publication. This work was supported by a grant from the Department of Energy, DE-FG03-89ER60891. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
Department of Energy (DOE)DE-FG03-89ER60891
Subject Keywords:electroporation; physical mapping; human genome
Issue or Number:18
PubMed Central ID:PMC50007
Record Number:CaltechAUTHORS:20141124-133258486
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Official Citation:Shizuya, H., Birren, B., Kim, U. J., Mancino, V., Slepak, T., Tachiiri, Y., & Simon, M. (1992). Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proceedings of the National Academy of Sciences, 89(18), 8794-8797. doi: 10.1073/pnas.89.18.8794
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52111
Deposited On:26 Nov 2014 15:29
Last Modified:10 Nov 2021 19:21

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