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A possible role for kinase-associated protein phosphatase in the Arabidopsis CLAVATA1 signaling  pathway

Williams, Robert W. and Wilson, Jeanne M. and Meyerowitz, Elliot M. (1997) A possible role for kinase-associated protein phosphatase in the Arabidopsis CLAVATA1 signaling  pathway. Proceedings of the National Academy of Sciences of the United States of America, 94 (19). pp. 10467-10472. ISSN 0027-8424. PMCID PMC23386. https://resolver.caltech.edu/CaltechAUTHORS:20141202-132511436

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Abstract

Continuous growth and development in plants are accomplished by meristems, groups of undifferentiated cells that persist as stem cells and initiate organs. While the structures of the apical and floral meristems in dicotyledonous plants have been well described, little is known about the underlying molecular mechanisms controlling cell proliferation and differentiation in these structures. We have shown previously that the CLAVATA1 (CLV1) gene in Arabidopsis encodes a receptor kinase-like protein that controls the size of the apical and floral meristems. Here, we show that KAPP, a gene encoding a kinase-associated protein phosphatase, is expressed in apical and young floral meristems, along with CLV1. Overexpression of KAPP mimics the clv1 mutant phenotype. Furthermore, CLV1 has kinase activity: it phosphorylates both itself and KAPP. Finally, KAPP binds and dephosphorylates CLV1. We present a model where KAPP functions as a negative regulator of the CLAVATA1 signal transduction pathway.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.94.19.10467 DOIArticle
http://www.pnas.org/content/94/19/10467PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC23386/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1997 The National Academy of Sciences. Contributed by Elliot M. Meyerowitz, July 23, 1997. We thank Hajime Sakai for assistance with the in situ hybridizations, Doris Wagner for advice on protein purification, and Akiko Kumagai for assistance with the phosphoamino acid analysis. We acknowledge Ray Deshaies and Renny Feldman for discussions, comments, and reagents. We also thank Xuemei Chen, Bill Dunphy, Jennifer Fletcher, Jian Hua, Toshiro Ito, Steve Jacobsen, Carolyn Ohno, Jose-Luis Reichmann, Mark Running, Robert Sablowski, Hajime Sakai, Tom Tubman, and Eva Ziegelhoffer for careful review of this manuscript. This work was supported by National Science Foundation Grant MCB-9204839 and a Strategic Research Fund Grant from Zeneca Seeds (to E.M.M). R.W.W. was supported by National Institutes of Health Predoctoral Training Grant GM07616 and the Howard Hughes Medical Institute. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked ‘‘advertisement’’ in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NSFMCB-9204839
Zeneca SeedsUNSPECIFIED
NIH Predoctoral FellowshipGM07616
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:meristem development; protein kinase; signal transduction; negative regulator
Issue or Number:19
PubMed Central ID:PMC23386
Record Number:CaltechAUTHORS:20141202-132511436
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141202-132511436
Official Citation:Robert W. Williams, Jeanne M. Wilson, and Elliot M. Meyerowitz A possible role for kinase-associated protein phosphatase in the Arabidopsis CLAVATA1 signaling pathway PNAS 1997 94 (19) 10467-10472
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52279
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:02 Dec 2014 21:31
Last Modified:03 Oct 2019 07:40

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