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Light-Induced Structural Changes in Photosynthetic Reaction Center: Implications for Mechanism of Electron-Proton Transfer

Stowell, M. H. B. and McPhillips, T. M. and Rees, D. C. and Soltis, S. M. and Abresch, E. and Feher, G. (1997) Light-Induced Structural Changes in Photosynthetic Reaction Center: Implications for Mechanism of Electron-Proton Transfer. Science, 276 (5313). pp. 812-816. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20141203-081101674

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Abstract

High resolution x-ray diffraction data from crystals of theRhodobacter sphaeroides photosynthetic reaction center (RC) have been collected at cryogenic temperature in the dark and under illumination, and the structures were refined at 2.2 and 2.6 angstrom resolution, respectively. In the charge-separated D+Q_AQ_B− state (where D is the primary electron donor (a bacteriochlorophyll dimer), and Q_A and Q_B are the primary and secondary quinone acceptors, respectively), Q_B− is located approximately 5 angstroms from the Q_B position in the charge-neutral (DQ_AQ_B) state, and has undergone a 180° propeller twist around the isoprene chain. A model based on the difference between the two structures is proposed to explain the observed kinetics of electron transfer from Q_A–Q_B to Q_AQ_B− and the relative binding affinities of the different ubiquinone species in the Q_B pocket. In addition, several water channels (putative proton pathways) leading from the Q_B pocket to the surface of the RC were delineated, one of which leads directly to the membrane surface.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.276.5313.812DOIArticle
http://www.sciencemag.org/content/276/5313/812PublisherArticle
ORCID:
AuthorORCID
Rees, D. C.0000-0003-4073-1185
Additional Information:© 1997 American Association for the Advancement of Science. Received 18 September 1996; accepted 10 March 1997. We thank M. Y. Okamura, M. L. Paddock, H. L. Axelrod, and M. S. Graige for insightful comments and discussions. This work was supported by grants from the National Institutes of Health (NIH GM13191, NIH GM45162). The rotation camera facility at SSRL is supported by the Department of Energy and by NIH. X-PLOR calculations were performed at the San Diego Supercomputer Center supported by NSF. The coordinates have been deposited in the Brookhaven Protein Data Bank with accession number 1aig and aij.
Funders:
Funding AgencyGrant Number
NIHGM13191
NIHGM45162
Department of Energy (DOE)UNSPECIFIED
NSFUNSPECIFIED
Issue or Number:5313
Record Number:CaltechAUTHORS:20141203-081101674
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141203-081101674
Official Citation:Light-Induced Structural Changes in Photosynthetic Reaction Center: Implications for Mechanism of Electron-Proton Transfer M. H. B. Stowell, T. M. McPhillips, D. C. Rees, S. M. Soltis, E. Abresch, and G. Feher Science 2 May 1997: 276 (5313), 812-816. [DOI:10.1126/science.276.5313.812]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52301
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Dec 2014 20:58
Last Modified:03 Oct 2019 07:41

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