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Mapping the protein regions responsible for the functional specificities of the Arabidopsis MADS domain organ-identity proteins

Krizek, Beth Allyn and Meyerowitz, Elliot M. (1996) Mapping the protein regions responsible for the functional specificities of the Arabidopsis MADS domain organ-identity proteins. Proceedings of the National Academy of Sciences of the United States of America, 93 (9). pp. 4063-4070. ISSN 0027-8424. PMCID PMC39487. http://resolver.caltech.edu/CaltechAUTHORS:20141203-125405575

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Abstract

The Arabidopsis MADS domain proteins AP1, AP3, PI, and AG specify floral organ identity. All of these proteins contain a MADS domain required for DNA binding and dimerization; a region termed L (linker between MADS domain and K domain), which plays an important role in dimerization specificity; the K domain, named for its similarity to the coiled-coil domain of keratin; and a C-terminal region of unknown function. To determine which regions of these proteins are responsible for their abilities to specify different organs, we have made a number of chimeric MADS box genes. The in vivo function of these chimeric genes was investigated by ectopic expression in transgenic Arabidopsis plants. The four proteins fall into two classes on the basis of regions responsible for their functional specificities. The L region and K domain define the functional specificities of AP3 and PI, while the MADS domain and L region define the functional specificities of AP1 and AG.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.93.9.4063 DOIArticle
http://www.pnas.org/content/93/9/4063PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC39487/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1996 National Academy of Sciences. Contributed by Elliot M. Meyerowitz, February 9, 1996. This contribution is part of the special series of Inaugural Articles by members of the National Academy of Sciences elected on April 25, 1995. We thank José Luis Riechmann, Yukiko Mizukami, and Hong Ma for allowing us to mention their unpublished results; Esther Koh for help with the Arabidopsis transformations; Detlef Weigel, Paul Stemberg, Tom Jack, and members of the laboratory for comments on the manuscript; and Pat Koen for advice on the use of the scanning electron microscope. B.A.K. was supported by American Cancer Society Postdoctoral Fellowship PF-3911. This work was supported by National Institutes of Health Grant GM45697 and U.S. National Science Foundation Grant MCB-9204839 to E.M.M.
Funders:
Funding AgencyGrant Number
American Cancer SocietyPF-3911
NIHGM45697
NSFMCB-9204839
Subject Keywords:flower development; domain swapping
PubMed Central ID:PMC39487
Record Number:CaltechAUTHORS:20141203-125405575
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20141203-125405575
Official Citation:B A Krizek and E M Meyerowitz Mapping the protein regions responsible for the functional specificities of the Arabidopsis MADS domain organ-identity proteins PNAS 1996 93 (9) 4063-4070
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52335
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Dec 2014 20:59
Last Modified:10 Dec 2016 18:25

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