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Dimerization specificity of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA, and AGAMOUS

Riechmann, José Luis and Krizek, Beth Allyn and Meyerowitz, Elliot M. (1996) Dimerization specificity of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA, and AGAMOUS. Proceedings of the National Academy of Sciences of the United States of America, 93 (10). pp. 4793-4798. ISSN 0027-8424. PMCID PMC39358. http://resolver.caltech.edu/CaltechAUTHORS:20141203-131723690

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Abstract

The MADS domain homeotic proteins APETALA1 (AP1), APETALA3 (AP3), PISTILLATA (PI), and AGAMOUS (AG) act in a combinatorial manner to specify the identity of Arabidopsis floral organs. The molecular basis for this combinatorial mode of action was investigated. Immunoprecipitation experiments indicate that all four proteins are capable of interacting with each other. However, these proteins exhibit "partner-specificity" for the formation of DNA-binding dimers; only AP1 homodimers, AG homodimers, and AP3/PI heterodimers are capable of binding to CArG-box sequences. Both the AP3/PI heterodimer and the AP1 or AG homodimers are formed when the three corresponding proteins are present together. The use of chimeric proteins formed by domain swapping indicates that the L region (which follows the MADS box) constitutes a key molecular determinant for the selective formation of DNA-binding dimers. The implications of these results for the ABC genetic model of flower development are discussed.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.93.10.4793 DOIArticle
http://www.pnas.org/content/93/10/4793PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC39358/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 1996 National Academy of Sciences. Contributed by Elliot M. Meyerowitz, January 18, 1996. We are grateful to Xuemei Chen, Jennifer Fletcher, Jian Hua, Steve Jacobsen, Joshua Levin, Zhongchi Liu, and Hajime Sakai for comments on the manuscript, and to Carl Parker for valuable discussions and suggestions. This work was supported by National Science Foundation Grant MCB-9204839 (E.M.M.). J.L.R. was a recipient of an European Molecular Biology Organization postdoctoral fellowship and is now supported by a fellowship from Ministerio de Educación y Ciencia (Spain). B.A.K. was supported by American Cancer Society postdoctoral fellowship PF-3911. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NSFMCB-9204839
European Molecular Biology Organization (EMBO)UNSPECIFIED
Ministerio de Educación y Ciencia (MEC)UNSPECIFIED
American Cancer SocietyPF-3911
Subject Keywords:flower development; ABC model; DNA binding; CArG box
PubMed Central ID:PMC39358
Record Number:CaltechAUTHORS:20141203-131723690
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20141203-131723690
Official Citation:J L Riechmann, B A Krizek, and E M Meyerowitz Dimerization specificity of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA, and AGAMOUS PNAS 1996 93 (10) 4793-4798
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52338
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Dec 2014 21:27
Last Modified:10 Dec 2016 18:28

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