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Developmental expression of synthetic cis-regulatory systems composed of spatial control elements from two different genes

Kirchhamer, Carmen V. and Bogarad, Leonard D. and Davidson, Eric H. (1996) Developmental expression of synthetic cis-regulatory systems composed of spatial control elements from two different genes. Proceedings of the National Academy of Sciences of the United States of America, 93 (24). pp. 13849-13854. ISSN 0027-8424. PMCID PMC19445. http://resolver.caltech.edu/CaltechAUTHORS:20141203-134524672

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Abstract

Synthetic cis-regulatory systems consisting of positively and negatively acting cis-regulatory modules of the Endo16 gene were combined with the lineage-specific regulatory element of the SM50 gene associated with a reporter and injected into eggs of sea urchins. We show here that synthetic cis-regulatory systems consisting of the positive Endo16 regulatory elements linked with the SM50 regulatory element are expressed spatially exactly as the sum of the individual endodermal and skeletogenic expression patterns. In combination, both lineage-specific positive regulatory elements function autonomously. However, addition of the Endo16 regulatory module that represses ectopic skeletogenic expression of Endo16 receptor constructs does not affect expression driven by the SM50 regulatory elements in the same skeletogenic cells. The repression function of this element is thus dedicated to control of the positive spatial output of the Endo16 regulatory system.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.93.24.13849DOIArticle
http://www.pnas.org/content/93/24/13849PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC19445/PubMed CentralArticle
Additional Information:© 1996 The National Academy of Sciences. Accepted September 9, 1996. We thank Dr. Steve Small of New York University and Drs. Ellen Rothenberg and James A. Coffman of the California Institute of Technology for reviewing this manuscript. This work was supported by National Institutes of Health Grant HD-05753 to E.H.D. and a Gosney Fellowship to L.D.B. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked ‘‘advertisement’’ in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHHD-05753
Caltech Gosney FellowshipUNSPECIFIED
Subject Keywords:transcriptional control; regulatory modules; Endo16; SM50
PubMed Central ID:PMC19445
Record Number:CaltechAUTHORS:20141203-134524672
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20141203-134524672
Official Citation:Kirchhamer, C. V., Bogarad, L. D., & Davidson, E. H. (1996). Developmental expression of synthetic cis-regulatory systems composed of spatial control elements from two different genes. Proceedings of the National Academy of Sciences, 93(24), 13849-13854.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52344
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:04 Dec 2014 18:09
Last Modified:23 Dec 2015 22:30

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