Kirchhamer, Carmen V. and Bogarad, Leonard D. and Davidson, Eric H. (1996) Developmental expression of synthetic cis-regulatory systems composed of spatial control elements from two different genes. Proceedings of the National Academy of Sciences of the United States of America, 93 (24). pp. 13849-13854. ISSN 0027-8424. PMCID PMC19445. doi:10.1073/pnas.93.24.13849. https://resolver.caltech.edu/CaltechAUTHORS:20141203-134524672
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Abstract
Synthetic cis-regulatory systems consisting of positively and negatively acting cis-regulatory modules of the Endo16 gene were combined with the lineage-specific regulatory element of the SM50 gene associated with a reporter and injected into eggs of sea urchins. We show here that synthetic cis-regulatory systems consisting of the positive Endo16 regulatory elements linked with the SM50 regulatory element are expressed spatially exactly as the sum of the individual endodermal and skeletogenic expression patterns. In combination, both lineage-specific positive regulatory elements function autonomously. However, addition of the Endo16 regulatory module that represses ectopic skeletogenic expression of Endo16 receptor constructs does not affect expression driven by the SM50 regulatory elements in the same skeletogenic cells. The repression function of this element is thus dedicated to control of the positive spatial output of the Endo16 regulatory system.
Item Type: | Article | ||||||||||||
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Additional Information: | © 1996 The National Academy of Sciences. Accepted September 9, 1996. We thank Dr. Steve Small of New York University and Drs. Ellen Rothenberg and James A. Coffman of the California Institute of Technology for reviewing this manuscript. This work was supported by National Institutes of Health Grant HD-05753 to E.H.D. and a Gosney Fellowship to L.D.B. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked ‘‘advertisement’’ in accordance with 18 U.S.C. §1734 solely to indicate this fact. | ||||||||||||
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Subject Keywords: | transcriptional control; regulatory modules; Endo16; SM50 | ||||||||||||
Issue or Number: | 24 | ||||||||||||
PubMed Central ID: | PMC19445 | ||||||||||||
DOI: | 10.1073/pnas.93.24.13849 | ||||||||||||
Record Number: | CaltechAUTHORS:20141203-134524672 | ||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20141203-134524672 | ||||||||||||
Official Citation: | Kirchhamer, C. V., Bogarad, L. D., & Davidson, E. H. (1996). Developmental expression of synthetic cis-regulatory systems composed of spatial control elements from two different genes. Proceedings of the National Academy of Sciences, 93(24), 13849-13854. | ||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
ID Code: | 52344 | ||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||
Deposited By: | INVALID USER | ||||||||||||
Deposited On: | 04 Dec 2014 18:09 | ||||||||||||
Last Modified: | 10 Nov 2021 19:24 |
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