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A multimerizing transcription factor of sea urchin embryos capable of looping DNA

Zeller, Robert W. and Griffith, Jack D. and Moore, James G. and Kirchhamer, Carmen V. and Britten, Roy J. and Davidson, Eric H. (1995) A multimerizing transcription factor of sea urchin embryos capable of looping DNA. Proceedings of the National Academy of Sciences of the United States of America, 92 (7). pp. 2989-2993. ISSN 0027-8424. PMCID PMC42344. https://resolver.caltech.edu/CaltechAUTHORS:20141203-135120757

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Abstract

SpGCF1 is a recently cloned sea urchin transcription factor that recognizes target sites in several different sea urchin genes. We find that in gel-shift experiments this factor is able to multimerize. A quantitative simulation of the gel-shift results suggests that SpGCF1 molecules that are bound to DNA target sites may also bind to one another, thus associating several DNA probe molecules. SpGCF1 might therefore be able to loop DNA molecules bearing its target sites at distant locations. We demonstrate this prediction by electron microscopy, and using the well-characterized cis-regulatory domain of the CyIIIa cytoskeletal actin gene, we show that the loop conformations predicted from the known SpGCF1 target site locations are actually formed in vitro. We speculate that the multimerization of this factor in vivo may function to bring distant regions of extended regulatory domains into immediate proximity so that they can interact with one another.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.92.7.2989DOIArticle
http://www.pnas.org/content/92/7/2989PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC42344/PubMed CentralArticle
Additional Information:© 1995 National Academy of Sciences. Contributed by Eric H. Davidson, December 20, 1994. We are pleased to acknowledge the useful insight of Prof. Robert Tjian, who in a telephone conversation early in this project alerted us to relevant studies on Spl carried out in his laboratory (21, 25). We are grateful to Profs. Carl Parker and Scott Fraser for their critical reviews of this manuscript and to Ms. Lora Cavallo for help with the EM preparations. This work was supported by National Institutes of Health Grants HD-05753 to E.H.D. and GM-31839 and GM-42342 to J.D.G. R.W.Z. was supported by an Office of Naval Research Augmentation Awards for Science and Engineering Research Training program (N00014-93-1-1400) and C.V.K. was supported by a training grant from the Austrian government.
Funders:
Funding AgencyGrant Number
NIHHD-05753
NIHGM-31839
NIHGM-42342
Office of Naval Research (ONR)UNSPECIFIED
Austrian governmentUNSPECIFIED
Issue or Number:7
PubMed Central ID:PMC42344
Record Number:CaltechAUTHORS:20141203-135120757
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141203-135120757
Official Citation:Zeller, R. W., Griffith, J. D., Moore, J. G., Kirchhamer, C. V., Britten, R. J., & Davidson, E. H. (1995). A multimerizing transcription factor of sea urchin embryos capable of looping DNA. Proceedings of the National Academy of Sciences, 92(7), 2989-2993. doi: 10.1073/pnas.92.7.2989
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52346
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:03 Dec 2014 23:55
Last Modified:03 Oct 2019 07:41

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