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Separation of the arterial wall from blood contact using hydrogel barriers reduces intimal thickening after balloon injury in the rat: The roles of medial and luminal factors in arterial healing

West, Jennifer L. and Hubbell, Jeffrey A. (1996) Separation of the arterial wall from blood contact using hydrogel barriers reduces intimal thickening after balloon injury in the rat: The roles of medial and luminal factors in arterial healing. Proceedings of the National Academy of Sciences of the United States of America, 93 (23). pp. 13188-13193. ISSN 0027-8424. PMCID PMC24068. http://resolver.caltech.edu/CaltechAUTHORS:20141204-113249788

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Abstract

The objective of this study was to clarify the relative roles of medial versus luminal factors in the induction of thickening of the arterial intima after balloon angioplasty injury. Platelet-derived growth factor (PDGF) and thrombin, both associated with thrombosis, and basic fibroblast growth factor (bFGF), stored in the arterial wall, have been implicated in this process. To unequivocally isolate the media from luminally derived factors, we used a 20-μm thick hydrogel barrier that adhered firmly to the arterial wall to block thrombus deposition after balloon-induced injury of the carotid artery of the rat. Thrombosis, bFGF mobilization, medial repopulation, and intimal thickening were measured. Blockade of postinjury arterial contact with blood prevented thrombosis and dramatically inhibited both intimal thickening and endogenous bFGF mobilization. By blocking blood contact on the two time scales of thrombosis and of intimal thickening, and by using local protein release to probe, by reconstitution, the individual roles of PDGF-BB and thrombin, we were able to conclude that a luminally derived factor other than PDGF or thrombin is required for the initiation of cellular events leading to intimal thickening after balloon injury in the rat. We further conclude that a luminally derived factor is required for mobilization of medial bFGF.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.pnas.org/content/93/23/13188PublisherArticle
http://dx.doi.org/10.1073/pnas.93.23.13188DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC24068/PubMed CentralArticle
Additional Information:© 1996 National Academy of Sciences. Communicated by Norman Davidson, California Institute of Technology, Pasadena, CA, August 23, 1996 (received for review May 31, 1996). The technical assistance of Kathleen Luther and Valerie Virta is greatly appreciated. We thank Focal, Inc. (Lexington, MA) for partial research funding.
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Funding AgencyGrant Number
Focal, Inc.UNSPECIFIED
PubMed Central ID:PMC24068
Record Number:CaltechAUTHORS:20141204-113249788
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20141204-113249788
Official Citation:Jennifer L. West and Jeffrey A. Hubbell Separation of the arterial wall from blood contact using hydrogel barriers reduces intimal thickening after balloon injury in the rat: The roles of medial and luminal factors in arterial healing PNAS 1996 93 (23) 13188-13193
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52392
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:04 Dec 2014 20:03
Last Modified:20 Jul 2017 19:40

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