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Isolation and characterization of a full-length cDNA coding for an adipose differentiation-related protein

Jiang, Hui-Ping and Serrero, Ginette (1992) Isolation and characterization of a full-length cDNA coding for an adipose differentiation-related protein. Proceedings of the National Academy of Sciences of the United States of America, 89 (17). pp. 7856-7860. ISSN 0027-8424. PMCID PMC49813. doi:10.1073/pnas.89.17.7856.

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We have previously isolated from a 1246 adipocyte cDNA library a cDNA clone called 154, corresponding to a mRNA that increases abundantly at a very early time during the differentiation of 1246 adipocytes and in adipocyte precursors in primary culture. We show here that the mRNA encoded by this cDNA is expressed abundantly and preferentially in mouse fat pads. A full-length cDNA for clone 154 was isolated by the RACE (rapid amplification of cDNA ends) protocol. Sequence analysis of this cDNA indicates that it encodes a protein of the 425 amino acids [tentatively named adipose differentiation-related protein (ADRP)] that does not have any similarity with sequences contained in the GenBank DNA and Protein Identification Resource protein data bases. Immunoblot of 1246 cell extracts with an antibody raised against the expressed ADRP shows that the 1246 cells contain a 50-kDa protein, the production of which increases as the cells differentiate. Localization of ADRP in 1246 cells indicates that ADRP is absent from nuclear and cytosolic fractions and is found as a membrane-associated protein. These results demonstrate that adipocyte differentiation is accompanied by early expression of a mRNA encoding a membrane-associated adipose differentiation related protein that is adipose tissue specific in vivo.

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Additional Information:© 1992 National Academy of Sciences. Communicated by Gordon H. Sato, April 22, 1992. We wish to thank Drs. Stephen Harris, Jun Hayashi, and Daniel Sussman for helpful advice and critical reading of the manuscript; Carol Baine and Marina LaDuke for the illustrations; and Valerie Oliver for preparation of the manuscript. This work was supported by Grant DK38639 from the National Institutes of Health and Grant 2003 from the Council for Tobacco Research. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
Council for Tobacco Research2003
Subject Keywords:obesity; adipocyte; adipose tissue
Issue or Number:17
PubMed Central ID:PMC49813
Record Number:CaltechAUTHORS:20141205-135304004
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Official Citation:H P Jiang and G Serrero Isolation and characterization of a full-length cDNA coding for an adipose differentiation-related protein PNAS 1992 89 (17) 7856-7860
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52440
Deposited By: Tony Diaz
Deposited On:10 Dec 2014 17:22
Last Modified:10 Nov 2021 19:41

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