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Optical Triggers of Protein Folding

Chan, Chi-Kin and Hofrichter, James and Eaton, William A. and Winkler, Jay R. and Gray, Harry B. (1996) Optical Triggers of Protein Folding. Science, 274 (5287). p. 629. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20141208-101657088

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Abstract

In our report about the electron-transfer (ET)-initiated folding of ferrocytochrome c (cyt c^(II)) (1), we noted rapid changes in the visible absorption spectrum corresponding to a process with a time constant of ~40 µs. We indicated that this observation was consistent with studies of cyt c^(ll) folding initiated by CO dissociation where the fast dynamics were attributed to changes in heme ligation (2). We also suggested that these dynamics might correspond to the collapse of the protein into a compact denatured state, as has been proposed for apomyoglobinon the basis of laser-temperature-jump measurements (3). Our transient absorption data could not distinguish between the two possibilities, and these fast folding dynamics were a relatively minor component of the study described in our report. Chan et al., with the use of tryptophan (Trp) fluorescence as a probe, found no significant collapse of the protein on the submillisecond time scale following dissociation of CO from unfolded cyt c^(II). Clearly, multiples pectroscopic probes must be employed to study protein folding; accordingly, we are currently developing time-resolved Trp fluorescence as a probe for ET-initiated folding of cyt c^(II).


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.jstor.org/stable/2899645JSTORArticle
http://dx.doi.org/10.1126/science.274.5287.628DOIArticle
http://www.sciencemag.org/content/274/5287/628PublisherArticle
ORCID:
AuthorORCID
Winkler, Jay R.0000-0002-4453-9716
Additional Information:© 1996 American Association for the Advancement of Science. Received 15 August 1996; accepted 10 September 1996.
Record Number:CaltechAUTHORS:20141208-101657088
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20141208-101657088
Official Citation:Optical Triggers of Protein Folding C.-K. Chan, J. Hofrichter, W. A. Eaton, J. R. Winkler, and H. B. Gray Science 25 October 1996: 274 (5287), 628-629. [DOI:10.1126/science.274.5287.628]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52463
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 Dec 2014 21:45
Last Modified:20 Sep 2017 23:25

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