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Lensfree optofluidic plasmonic sensor for real-time and label-free monitoring of molecular binding events over a wide field-of-view

Coskun, Ahmet F. and Cetin, Arif E. and Galarreta, Betty C. and Alvarez, Daniel Adrianzen and Altug, Hatice and Ozcan, Aydogan (2014) Lensfree optofluidic plasmonic sensor for real-time and label-free monitoring of molecular binding events over a wide field-of-view. Scientific Reports, 4 (10). Art. No. 6789. ISSN 2045-2322. PMCID PMC4209447.

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We demonstrate a high-throughput biosensing device that utilizes microfluidics based plasmonic microarrays incorporated with dual-color on-chip imaging toward real-time and label-free monitoring of biomolecular interactions over a wide field-of-view of >20 mm^2. Weighing 40 grams with 8.8 cm in height, this biosensor utilizes an opto-electronic imager chip to record the diffraction patterns of plasmonic nanoapertures embedded within microfluidic channels, enabling real-time analyte exchange. This plasmonic chip is simultaneously illuminated by two different light-emitting-diodes that are spectrally located at the right and left sides of the plasmonic resonance mode, yielding two different diffraction patterns for each nanoaperture array. Refractive index changes of the medium surrounding the near-field of the nanostructures, e.g., due to molecular binding events, induce a frequency shift in the plasmonic modes of the nanoaperture array, causing a signal enhancement in one of the diffraction patterns while suppressing the other. Based on ratiometric analysis of these diffraction images acquired at the detector-array, we demonstrate the proof-of-concept of this biosensor by monitoring in real-time biomolecular interactions of protein A/G with immunoglobulin G (IgG) antibody. For high-throughput on-chip fabrication of these biosensors, we also introduce a deep ultra-violet lithography technique to simultaneously pattern thousands of plasmonic arrays in a cost-effective manner.

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Coskun, Ahmet F.0000-0002-5797-1524
Additional Information:© 2014 Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. Received 28 July 2014. Accepted 6 October 2014. Published 27 October 2014. Altug Research Group acknowledges National Science Foundation (NSF) CAREER Award, Presidential Early Career Award for Scientist and Engineers (PECASE) ECCS-0954790, Office of Naval Research Young Investigator Award 11PR00755-00-P00001, NSF Engineering Research Center on Smart Lighting EEC-0812056, Massachusetts Life Sciences Center Young Investigator award and Ecole Polytechnique Federale de Lausanne. Ozcan Research Group at UCLA gratefully acknowledges the support of the Presidential Early Career Award for Scientists and Engineers (PECASE), Army Research Office (ARO) Life Sciences Division (ARO; W911NF-13-1-0419 and W911NF-13-1-0197), ARO Young Investigator Award, National Science Foundation (NSF) CAREER Award, NSF CBET Division Biophotonics Program, NSF Emerging Frontiers in Research and Innovation (EFRI) Award, NSF EAGER Award, Office of Naval Research (ONR), the Howard Hughes Medical Institute (HHMI), and National Institutes of Health (NIH) Director’s New Innovator Award DP2OD006427 from the Office of the Director, National Institutes of Health. This work is partially based upon research performed in a renovated laboratory by the National Science Foundation under Grant No. 0963183, which is an award funded under the American Recovery and Reinvestment Act of 2009 (ARRA). The authors also thank UCSB for their contribution in fabrication of the nanoapertures.
Funding AgencyGrant Number
Office of Naval Research (ONR)11PR00755-00-P00001
Massachusetts Life Sciences CenterUNSPECIFIED
Ecole Polytechnique Federale de LausanneUNSPECIFIED
Army Research Office (ARO)W911NF-13-1-0419
Army Research Office (ARO)W911NF-13-1-0197
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
American Recovery and Reinvestment Act of 2009 (ARRA)0963183
Issue or Number:10
PubMed Central ID:PMC4209447
Record Number:CaltechAUTHORS:20141208-101947905
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Official Citation:Lensfree optofluidic plasmonic sensor for real-time and label-free monitoring of molecular binding events over a wide field-of-view Ahmet F. Coskun, Arif E. Cetin, Betty C. Galarreta, Daniel Adrianzen Alvarez, Hatice Altug & + et al. Scientific Reports 4, doi:10.1038/srep06789
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52464
Deposited By: Ruth Sustaita
Deposited On:09 Dec 2014 21:42
Last Modified:03 Oct 2019 07:42

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