Silipo, Alba and Vitiello, Giuseppe and Gully, Djamel and Sturiale, Luisa and Chaintreuil, Clémence and Fardoux, Joel and Gargani, Daniel and Lee, Hae-In and Kulkarni, Gargi and Busset, Nicolas and Marchetti, Roberta and Palmigiano, Angelo and Moll, Herman and Engel, Regina and Lanzetta, Rosa and Paduano, Luigi and Parrilli, Michelangelo and Chang, Woo-Suk and Holst, Otto and Newman, Dianne K. and Garozzo, Domenico and D'Errico, Eric and Giraud, Eric and Molinaro, Antonio (2014) Covalently linked hopanoid-lipid A improves outer-membrane resistance of a Bradyrhizobium symbiont of legumes. Nature Communications, 5 (10). Art. No. 5106. ISSN 2041-1723. doi:10.1038/ncomms6106. https://resolver.caltech.edu/CaltechAUTHORS:20141208-104816963
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Abstract
Lipopolysaccharides (LPSs) are major components of the outer membrane of Gram-negative bacteria and are essential for their growth and survival. They act as a structural barrier and play an important role in the interaction with eukaryotic hosts. Here we demonstrate that a photosynthetic Bradyrhizobium strain, symbiont of Aeschynomene legumes, synthesizes a unique LPS bearing a hopanoid covalently attached to lipid A. Biophysical analyses of reconstituted liposomes indicate that this hopanoid-lipid A structure reinforces the stability and rigidity of the outer membrane. In addition, the bacterium produces other hopanoid molecules not linked to LPS. A hopanoid-deficient strain, lacking a squalene hopene cyclase, displays increased sensitivity to stressful conditions and reduced ability to survive intracellularly in the host plant. This unusual combination of hopanoid and LPS molecules may represent an adaptation to optimize bacterial survival in both free-living and symbiotic states.
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Additional Information: | © 2014 Macmillan Publishers Limited. Received 17 Mar 2014; Accepted 29 Aug 2014; Published 30 Oct 2014. This work was supported by grants from the French national research agency (ANR-SESAM-2010-BLAN-170801 and ANR-BugsInaCell-13-BSV7-0013-02) from the Italian Ministry of Education, Universities and Research (PRIN 2009J98Z3_001, PRIN 2010 BJ23MN_007, PRIN 2011 L9SH3K and FIRB-MERIT RBNE08HWLZ), from Mizutani Foundation for Glycoscience 2014 and from grants from NASA (NNX12AD93G) and the Howard Hughes Medical Institute. D.K.N. is an HHMI Investigator. We thank J.F. Arrighi (IRD, France), A. Verméglio (CEA, Cadarache France), P. Mergaert and O. Pierre (ISV, CNRS, Gif-sur-Yvette, France), A. Imberty (Cermav, France) and T. Chrzanowski (UTA, Arlington, TX, USA) for criticisms and corrections of the manuscript. | ||||||||||||||||||||
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Subject Keywords: | Biological sciences; Biochemistry; Microbiology; Plant sciences | ||||||||||||||||||||
Issue or Number: | 10 | ||||||||||||||||||||
DOI: | 10.1038/ncomms6106 | ||||||||||||||||||||
Record Number: | CaltechAUTHORS:20141208-104816963 | ||||||||||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20141208-104816963 | ||||||||||||||||||||
Official Citation: | Silipo, A., Vitiello, G., Gully, D., Sturiale, L., Chaintreuil, C., Fardoux, J., . . . Molinaro, A. (2014). Covalently linked hopanoid-lipid A improves outer-membrane resistance of a Bradyrhizobium symbiont of legumes. [Article]. Nat Commun, 5. doi: 10.1038/ncomms6106 | ||||||||||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||||||||||
ID Code: | 52465 | ||||||||||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||||||||||
Deposited By: | Jason Perez | ||||||||||||||||||||
Deposited On: | 09 Dec 2014 21:27 | ||||||||||||||||||||
Last Modified: | 10 Nov 2021 19:41 |
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