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Covalently linked hopanoid-lipid A improves outer-membrane resistance of a Bradyrhizobium symbiont of legumes

Silipo, Alba and Vitiello, Giuseppe and Gully, Djamel and Sturiale, Luisa and Chaintreuil, Clémence and Fardoux, Joel and Gargani, Daniel and Lee, Hae-In and Kulkarni, Gargi and Busset, Nicolas and Marchetti, Roberta and Palmigiano, Angelo and Moll, Herman and Engel, Regina and Lanzetta, Rosa and Paduano, Luigi and Parrilli, Michelangelo and Chang, Woo-Suk and Holst, Otto and Newman, Dianne K. and Garozzo, Domenico and D'Errico, Eric and Giraud, Eric and Molinaro, Antonio (2014) Covalently linked hopanoid-lipid A improves outer-membrane resistance of a Bradyrhizobium symbiont of legumes. Nature Communications, 5 (10). Art. No. 5106. ISSN 2041-1723. http://resolver.caltech.edu/CaltechAUTHORS:20141208-104816963

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Abstract

Lipopolysaccharides (LPSs) are major components of the outer membrane of Gram-negative bacteria and are essential for their growth and survival. They act as a structural barrier and play an important role in the interaction with eukaryotic hosts. Here we demonstrate that a photosynthetic Bradyrhizobium strain, symbiont of Aeschynomene legumes, synthesizes a unique LPS bearing a hopanoid covalently attached to lipid A. Biophysical analyses of reconstituted liposomes indicate that this hopanoid-lipid A structure reinforces the stability and rigidity of the outer membrane. In addition, the bacterium produces other hopanoid molecules not linked to LPS. A hopanoid-deficient strain, lacking a ​squalene hopene cyclase, displays increased sensitivity to stressful conditions and reduced ability to survive intracellularly in the host plant. This unusual combination of hopanoid and LPS molecules may represent an adaptation to optimize bacterial survival in both free-living and symbiotic states.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/ncomms6106DOIArticle
http://www.nature.com/ncomms/2014/141030/ncomms6106/full/ncomms6106.htmlPublisherArticle
http://rdcu.be/cmnAPublisherFree ReadCube access
ORCID:
AuthorORCID
Newman, Dianne K.0000-0003-1647-1918
Additional Information:© 2014 Macmillan Publishers Limited. Received 17 Mar 2014; Accepted 29 Aug 2014; Published 30 Oct 2014. This work was supported by grants from the French national research agency (ANR-SESAM-2010-BLAN-170801 and ANR-BugsInaCell-13-BSV7-0013-02) from the Italian Ministry of Education, Universities and Research (PRIN 2009J98Z3_001, PRIN 2010 BJ23MN_007, PRIN 2011 L9SH3K and FIRB-MERIT RBNE08HWLZ), from Mizutani Foundation for Glycoscience 2014 and from grants from NASA (NNX12AD93G) and the Howard Hughes Medical Institute. D.K.N. is an HHMI Investigator. We thank J.F. Arrighi (IRD, France), A. Verméglio (CEA, Cadarache France), P. Mergaert and O. Pierre (ISV, CNRS, Gif-sur-Yvette, France), A. Imberty (Cermav, France) and T. Chrzanowski (UTA, Arlington, TX, USA) for criticisms and corrections of the manuscript.
Funders:
Funding AgencyGrant Number
Agence Nationale pour la Recherche (ANR) ANR-SESAM-2010-BLAN-170801
Agence Nationale pour la Recherche (ANR) ANR-BugsInaCell-13-BSV7-0013-02
Italian Ministry of Education, Universities and ResearchPRIN 2009J98Z3_001
Italian Ministry of Education, Universities and ResearchPRIN 2010 BJ23MN_007
Italian Ministry of Education, Universities and ResearchPRIN 2011 L9SH3K
Italian Ministry of Education, Universities and ResearchFIRB-MERIT RBNE08HWLZ
Mizutani Foundation for Glycoscience 2014UNSPECIFIED
NASANNX12AD93G
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:Biological sciences; Biochemistry; Microbiology; Plant sciences
Record Number:CaltechAUTHORS:20141208-104816963
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20141208-104816963
Official Citation:Silipo, A., Vitiello, G., Gully, D., Sturiale, L., Chaintreuil, C., Fardoux, J., . . . Molinaro, A. (2014). Covalently linked hopanoid-lipid A improves outer-membrane resistance of a Bradyrhizobium symbiont of legumes. [Article]. Nat Commun, 5. doi: 10.1038/ncomms6106
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52465
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:09 Dec 2014 21:27
Last Modified:24 Oct 2017 16:24

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