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A segment of the C-terminal half of the G-protein .31 subunit specifies its interaction with the γ4 subunit

Katz, Arieh and Simon, Melvin I. (1995) A segment of the C-terminal half of the G-protein .31 subunit specifies its interaction with the γ4 subunit. Proceedings of the National Academy of Sciences of the United States of America, 92 (6). pp. 1998-2002. ISSN 0027-8424. PMCID PMC42410. doi:10.1073/pnas.92.6.1998.

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The β and γ subunits of the heterotrimeric guanine nucleotide binding (G protein) act as a dimer and directly regulate various signal transduction pathways. By using cotransfection assays, we tested the ability of several βγ combinations to activate inositol phospholipid-specific phospholipase C (PI-PLC)-β2. Our findings indicate that only βγ combinations that form dimers will activate PI-PLC-beta 2. Since G beta 1 interacts with Gγ1, while G beta 2 cannot, chimeras between Gβ1 and Gβ2 were used to identify the regions in beta 1 that determine its specific association with γ1. Our evidence demonstrates that a chimera between β2 and β1 that contains the C-terminal 173 amino acids of beta 1 can interact and activate PI-PLC-β2 with γ1. Chimeras that contain portions of the β1 C-terminal region display a weaker association with γ1. Furthermore, the contribution of each of these regions depends on the sequence context of each chimeric protein. However, the segment between residues 210 and 293 of β1 consistently plays a critical role in specifying association with γ1.

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Katz, Arieh0000-0003-3487-7049
Additional Information:© 1994 National Academy of Sciences. Contributed by Melvin I. Simon, November 17, 1994. While this manuscript was under review, a paper by Garritsen and Simonds (39) that arrived at similiar conclusions by using different assay procedures appeared. We thank Heather Epstein for critical reading of the manuscript. This research was supported by National Institutes of Health Grant GM34236 (to M.I.S.).
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Issue or Number:6
PubMed Central ID:PMC42410
Record Number:CaltechAUTHORS:20141209-084231386
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Official Citation:Katz, A., & Simon, M. I. (1995). A segment of the C-terminal half of the G-protein beta 1 subunit specifies its interaction with the gamma 1 subunit. Proceedings of the National Academy of Sciences, 92(6), 1998-2002.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52489
Deposited By: Jason Perez
Deposited On:09 Dec 2014 21:44
Last Modified:10 Nov 2021 19:41

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