A Caltech Library Service

Strands hybridize in postreplicative adenovirus overlap recombination

Ahern, Kevin G. and Wang, Kai and Xu, Fu-Yun and Mathews, Catherine Z. and Pearson, George D. (1991) Strands hybridize in postreplicative adenovirus overlap recombination. Proceedings of the National Academy of Sciences of the United States of America, 88 (1). pp. 105-109. ISSN 0027-8424. PMCID PMC50758. doi:10.1073/pnas.88.1.105.

PDF - Published Version
See Usage Policy.


Use this Persistent URL to link to this item:


We describe a postreplicative mechanism for adenovirus overlap recombination. An adenovirus minichromosome system was used to study overlap recombination driven by adenovirus DNA replication. Crossing-over appeared to occur equally at, but not within, the borders of the overlap between partner molecules. We propose that recombination in the minichromosome system proceeds through an intermediate formed by direct hybridization of complementary sequences on displaced strands generated by adenovirus-specific DNA replication. Some, but not all, heterologous regions in the intermediate are susceptible to mismatch correction. This pathway is intrinsically nonreciprocal and differs significantly from other adenovirus recombinational mechanisms that have been described previously.

Item Type:Article
Related URLs:
URLURL TypeDescription DOIArticle CentralArticle
Additional Information:© 1991 National Academy of Sciences. Communicated by K. E. van Holde, October 4, 1990 (received for review September 1, 1990). We thank Dr. Kathleen L. Berkner for providing plasmid pIBIV used to make pXD6. This work was supported by National Institutes of Health Grant CA17699 (to G.D.P.) and American Cancer Society Grant PF-2938 (to K.G.A.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
American Cancer SocietyPF-2938
Subject Keywords:intermolecular reassociation; nonreciprocal recombination; mismatch correction; precise gene fusion
Issue or Number:1
PubMed Central ID:PMC50758
Record Number:CaltechAUTHORS:20141210-075806932
Persistent URL:
Official Citation:K G Ahern, K Wang, F Y Xu, C Z Mathews, and G D Pearson Strands hybridize in postreplicative adenovirus overlap recombination PNAS 1991 88 (1) 105-109
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52520
Deposited By: Tony Diaz
Deposited On:10 Dec 2014 18:13
Last Modified:10 Nov 2021 19:42

Repository Staff Only: item control page