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A cocaine-sensitive Drosophila serotonin transporter: Cloning, expression, and electrophysiological characterization

Corey, Janis L. and Quick, Michael W. and Davidson, Norman and Lester, Henry A. and Guastella, John (1994) A cocaine-sensitive Drosophila serotonin transporter: Cloning, expression, and electrophysiological characterization. Proceedings of the National Academy of Sciences of the United States of America, 91 (3). pp. 1188-1192. ISSN 0027-8424. PMCID PMC521479. https://resolver.caltech.edu/CaltechAUTHORS:20141210-095231086

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Abstract

A cocaine-sensitive, high-affinity Drosophila serotonin (5-hydroxytryptamine; 5HT) transporter cDNA, denoted dSERT1, was isolated and characterized in oocytes. dSERT1 shows little transport of other monoamines and is Na^+ and Cl^- dependent. Sequence analysis indicates 12 putative transmembrane domains and strong homologies (≈50%) among dSERT1 and mammalian 5HT, norepinephrine, and dopamine transporters. Interestingly, the pharmacological properties of dSERT1, including sensitivity to antidepressants, are more similar to those of mammalian catecholamine transporters than to mammalian 5HT transporters. Two-electrode voltage-clamp analysis demonstrated 5HT-induced, voltage-dependent currents. Cloning and characterization of dSERT1 adds significantly to our knowledge of the diversity of 5HT transporters with regard to primary sequence, pharmacological profile, and permeation properties.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC521479/PubMed CentralArticle
http://www.pnas.org/content/91/3/1188PublisherArticle
ORCID:
AuthorORCID
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 1993 National Academy of Sciences. Contributed by Norman Davidson, October 1, 1993. We thank Bruce Hamilton for providing us with the Drosophila head library, Jonathan Bradley for Drosophila embryo RNA, Susan R. Halsell for providing Drosophila for RNA preparation, and E. Davidson for use of a DNA sequenator. This research was supported by grants from the U.S. Public Health Service (Grant NS-11756 and National Research Service Award Fellowships to J.L.C., M.W.Q., and J.G.).
Funders:
Funding AgencyGrant Number
NIHNS-11756
NIH Postdoctoral FellowshipUNSPECIFIED
Issue or Number:3
PubMed Central ID:PMC521479
Record Number:CaltechAUTHORS:20141210-095231086
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141210-095231086
Official Citation:Corey, J. L., Quick, M. W., Davidson, N., Lester, H. A., & Guastella, J. (1994). A cocaine-sensitive Drosophila serotonin transporter: cloning, expression, and electrophysiological characterization. Proceedings of the National Academy of Sciences, 91(3), 1188-1192. doi: 10.1073/pnas.91.3.1188
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52541
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:10 Dec 2014 20:59
Last Modified:03 Oct 2019 07:43

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