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A cell cycle-responsive transcriptional control element and a negative control element in the gene encoding DNA polymerase alpha in Saccharomyces cerevisiae

Gordon, Colin B. and Campbell, Judith L. (1991) A cell cycle-responsive transcriptional control element and a negative control element in the gene encoding DNA polymerase alpha in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences of the United States of America, 88 (14). pp. 6058-6062. ISSN 0027-8424. https://resolver.caltech.edu/CaltechAUTHORS:20141210-104124068

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Abstract

Transcription of the POL1 gene of Saccharomyces cerevisiae, which encodes DNA polymerase alpha, the DNA polymerase required for the initiation of DNA replication, has previously been shown to be cell cycle regulated. To understand how the POL1 gene senses cell cycle position, we have investigated the cis-acting elements that respond to the factors that govern cell cycle progression. In this report we demonstrate that a region of 54 nucleotides containing the repeated element ACGCGT, which conforms to an Mlu I restriction endonuclease recognition site, contains all information necessary for transcriptional activation and cell cycle responsiveness. Although oligonucleotides lacking either one or both of the repeated Mlu I sites can function as an upstream activating sequence, the presence of at least one Mlu I site stimulates expression and, moreover, is absolutely essential for cell cycle regulation. A synthetic oligonucleotide corresponding to a 19-base-pair sequence in the POL1 promoter containing one Mlu I site can function as an autonomous cell cycle-responsive upstream element (upstream activation sequence) with temporal regulation indistinguishable from that previously described for the POL1 gene. Thus, the Mlu I site is an essential part of a cis-acting element responsible for the observed periodic activation. This sequence differs from previously defined cell cycle-responsive transcriptional control elements in the yeast HO endonuclease and histone genes. We also present evidence for a negative regulatory element in the 5' flanking region of the Mlu I upstream activation sequence.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.88.14.6058 DOIArticle
http://www.pnas.org/content/88/14/6058PublisherArticle
Additional Information:© 1991 National Academy of Sciences. Communicated by John Abelson, February 19, 1991. We thank Scott Moye-Rowley, Ambrose Jong, and Brenda Andrews for plasmids and strains. We thank Scott Moye-Rowley and members of the Campbell laboratory for many stimulating discussions throughout the course of this work and Nick Hastie for helpful comments on the manuscript. This work was supported by a grant from the National Institutes of Health (GM-25508) and a Procter and Gamble postdoctoral fellowship to C.B.G.
Funders:
Funding AgencyGrant Number
NIHGM-25508
Procter and GambleUNSPECIFIED
Subject Keywords:DNA replication; yeast; G1 phase
Issue or Number:14
Record Number:CaltechAUTHORS:20141210-104124068
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141210-104124068
Official Citation:C B Gordon and J L Campbell A cell cycle-responsive transcriptional control element and a negative control element in the gene encoding DNA polymerase alpha in Saccharomyces cerevisiae PNAS 1991 88 (14) 6058-6062
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52548
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 Dec 2014 21:03
Last Modified:03 Oct 2019 07:43

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